Peptidoglycan in osteoarthritis synovial tissue is associated with joint inflammation.

Autor: Holub MN; Department of Orthopaedic Surgery, Medical College of Wisconsin, BSB room 2850, Milwaukee, WI, 53226, USA.; Department of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, WI, USA., Wahhab A; Department of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, WI, USA., Rouse JR; Department of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, WI, USA., Danner R; Department of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, WI, USA., Hackner LG; Department of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, WI, USA., Duris CB; Department of Pathology, Children's Hospital of Wisconsin and the Medical College of Wisconsin, Milwaukee, WI, USA., McClune ME; Department of Biochemistry, Virginia Tech, Blacksburg, VA, USA.; Fralin Life Sciences Institute, Virginia Tech, Blacksburg, VA, USA.; Center for Emerging, Zoonotic, and Arthropod-borne Pathogens, Virginia Tech, Blacksburg, VA, USA., Dressler JM; Department of Biochemistry, Virginia Tech, Blacksburg, VA, USA.; Fralin Life Sciences Institute, Virginia Tech, Blacksburg, VA, USA., Strle K; Department of Molecular Biology and Microbiology, Tufts University, Boston, MA, USA., Jutras BL; Department of Biochemistry, Virginia Tech, Blacksburg, VA, USA.; Fralin Life Sciences Institute, Virginia Tech, Blacksburg, VA, USA.; Center for Emerging, Zoonotic, and Arthropod-borne Pathogens, Virginia Tech, Blacksburg, VA, USA., Edelstein AI; Department of Orthopaedic Surgery, Northwestern University Feinberg School of Medicine, Chicago, IL, USA. aedelst1@nm.org., Lochhead RB; Department of Orthopaedic Surgery, Medical College of Wisconsin, BSB room 2850, Milwaukee, WI, 53226, USA. rlochhead@mcw.edu.; Department of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, WI, USA. rlochhead@mcw.edu.; Division of Rheumatology, Department of Medicine, Medical College of Wisconsin, Milwaukee, WI, USA. rlochhead@mcw.edu.
Jazyk: angličtina
Zdroj: Arthritis research & therapy [Arthritis Res Ther] 2024 Mar 27; Vol. 26 (1), pp. 77. Date of Electronic Publication: 2024 Mar 27.
DOI: 10.1186/s13075-024-03293-x
Abstrakt: Objectives: Peptidoglycan (PG) is an arthritogenic bacterial cell wall component whose role in human osteoarthritis is poorly understood. The purpose of this study was to determine if PG is present in synovial tissue of osteoarthritis patients at the time of primary total knee arthroplasty (TKA), and if its presence is associated with inflammation and patient reported outcomes.
Methods: Intraoperative synovial tissue and synovial fluid samples were obtained from 56 patients undergoing primary TKA, none of whom had history of infection. PG in synovial tissue was detected by immunohistochemistry (IHC) and immunofluorescence microscopy (IFM). Synovial tissue inflammation and fibrosis were assessed by histopathology and synovial fluid cytokine quantification. Primary human fibroblasts isolated from arthritis synovial tissue were stimulated with PG to determine inflammatory cytokine response.
Results: A total of 33/56 (59%) of primary TKA synovial tissue samples were positive for PG by IHC, and PG staining colocalized with markers of synovial macrophages and fibroblasts by IFM. Synovial tissue inflammation and elevated IL-6 in synovial fluid positively correlated with PG positivity. Primary human fibroblasts stimulated with PG secreted high levels of IL-6, consistent with ex vivo findings. Interestingly, we observed a significant inverse correlation between PG and age at time of TKA, indicating younger age at time of TKA was associated with higher PG levels.
Conclusion: Peptidoglycan is commonly found in synovial tissue from patients undergoing TKA. Our data indicate that PG may play an important role in inflammatory synovitis, particularly in patients who undergo TKA at a relatively younger age.
(© 2024. The Author(s).)
Databáze: MEDLINE
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