Two-Dimensional Gel Electrophoresis in Studies of Flower and Leaf Proteome of Common Buckwheat.
| Autor: | Kopeć P; The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków, Poland. p.kopec@ifr-pan.edu.pl., Krzewska M; The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków, Poland., Płażek A; Department of Physiology, Breeding of Plant and Seed Science, University of Agriculture, Cracow, Poland. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2791, pp. 113-119. |
| DOI: | 10.1007/978-1-0716-3794-4_12 |
| Abstrakt: | Two-dimensional gel electrophoresis (2-DE) is a proteomic tool used for the separation of protein mixtures according to protein isoelectric point and molecular mass. Although gel-free quantitative and qualitative proteomic study techniques are now available, 2-DE remains a useful analytical tool. The presented protocol was performed to analyze the flower and leaf proteome of common buckwheat using 24 cm immobilized pH gradient strips (pH 4-7) and visualization of proteins on gels via colloidal Coomassie G-250 staining. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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