Phenol-Based Protein Extraction Method for Plant Proteomic Studies.
| Autor: | Kopeć P; The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków, Poland. p.kopec@ifr-pan.edu.pl., Krzewska M; The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków, Poland., Płażek A; Department of Physiology, Breeding of Plant and Seed Science, University of Agriculture, Cracow, Poland. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2791, pp. 107-111. |
| DOI: | 10.1007/978-1-0716-3794-4_11 |
| Abstrakt: | The protein extraction method based on the phenol solution and combined with protein precipitation with ammonium acetate in methanol and purification in the same solution, and additionally in acetone and ethanol, is recommended for proteomic studies of plant tissues. The obtained protein samples do not require additional nucleic acid digestion and removal of interfering contaminations. The presented protocol was used to analyze the proteome of common buckwheat flowers and leaves. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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