Biological effects and phytochemical study of the underground part of Iris scariosa Willd. ex Link extract: A new source of bioactive constituents.

Autor: Omarova BA; Asfendiyarov Kazakh National Medical University, Tole Bi St. 94, Almaty 050000, Republic of Kazakhstan., Shults EE; Novosibirsk Institute of Organic Chemistry, Siberian Branch of the Russian Academy of Sciences, Acad. Lavrentyev Ave. 9, 630090 Novosibirsk, Russia. Electronic address: schultz@nioch.nsc.ru., Zhakipbekov KS; Asfendiyarov Kazakh National Medical University, Tole Bi St. 94, Almaty 050000, Republic of Kazakhstan. Electronic address: zhakipbekov.k@kaznmu.kz., Abekova АО; JSC «Scientific Center for Anti-Infectious Drugs», al-Farabi Ave. 75A, 050060 Almaty, Republic of Kazakhstan., Ishmuratova MY; NCJSC 'Buketov Karaganda University', Universitetskaya Str., 28/3, 100028 Karaganda, Republic of Kazakhstan., Petrova TN; Novosibirsk Institute of Organic Chemistry, Siberian Branch of the Russian Academy of Sciences, Acad. Lavrentyev Ave. 9, 630090 Novosibirsk, Russia., Kartbayeva EB; Asfendiyarov Kazakh National Medical University, Tole Bi St. 94, Almaty 050000, Republic of Kazakhstan; Higher School of Medicine, Al-Farabi Kazakh National University, 71 al-Farabi Ave., Almaty 050040, Republic of Kazakhstan.
Jazyk: angličtina
Zdroj: Fitoterapia [Fitoterapia] 2024 Jun; Vol. 175, pp. 105920. Date of Electronic Publication: 2024 Mar 24.
DOI: 10.1016/j.fitote.2024.105920
Abstrakt: The expected toxicity and resistance of chemotherapeutic agents necessitate and encourage for the use of natural chemotherapeutic sources of plant origin in the clinical stage of cancer therapy. Plants of the genus Iris (Iridaceae) used by local populations for the treatment of cancer, bacterial and viral infections. In this study, an ethanol extract of rhizomes of I. scariosa was prepared and tested for the cytotoxicity using the MTT assay. The extract exhibited the most potent cytotoxicity against the breast cancer cell line MCF7 (IC 50  = 9.28 ± 0.49 μg/ml, selectively index ˃5), and induced apoptosis in MCF7 lines. Notably, the extract significantly inhibited the colony formation of MCF7 and HepG2 cancer cells at a concentration range from 10.6 to 85.0 μg/ml, including non-toxic concentrations for HepG2 cells. The ethanol extract was analyzed by HPLC, revealed the identification of 5 secondary metabolites (quercetin, rutin, myricetin, apigenin, artemisetin), the content of which was shown to reach around 15% of the extract. The petroleum ether (PE) part of the extract (yield 2.62%) was analyzed by GC-MS. The composition of tert-butyl methyl ether (TBME) part of the extract (yield 23.72%) was studied. Total of 15 individual compounds: two benzophenones, eight isoflavones, four flavones and a (2R)-flavanone were isolated. The pentamethoxyflavone artemisetin and flavanone pinocembrin were isolated for the first from Iris sp. The readily available isoflavones from the TBME part of extract (irilone, iriflogenin, irigenin and tectorigenin) may serve as new leads for the discovery of anticancer drugs.
Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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