Two lateral flow assays for detection of anti-coccidioidal antibodies show similar performance to immunodiffusion in dogs with coccidioidomycosis.

Autor:	Grill FJ; Cactus Bio, LLC, Phoenix, AZ.; Department of Laboratory Medicine and Pathology, Mayo Clinic, Phoenix, AZ., Jaffey JA; Department of Specialty Medicine, College of Veterinary Medicine, Midwestern University, Glendale, AZ., Grys TE; Cactus Bio, LLC, Phoenix, AZ.; Department of Laboratory Medicine and Pathology, Mayo Clinic, Phoenix, AZ., Rayhel LH; Department of Specialty Medicine, College of Veterinary Medicine, Midwestern University, Glendale, AZ., Lake DF; Cactus Bio, LLC, Phoenix, AZ.; School of Life Sciences, Arizona State University, Tempe, AZ.
Jazyk:	angličtina
Zdroj:	American journal of veterinary research [Am J Vet Res] 2024 Mar 25; Vol. 85 (6). Date of Electronic Publication: 2024 Mar 25 (Print Publication: 2024).
DOI:	10.2460/ajvr.23.12.0272
Abstrakt:	Objective: To compare 2 point-of-care lateral flow assays (LFAs) with immunodiffusion (ID) IgG results for anti-coccidioidal antibody detection in dogs with coccidioidomycosis. A further aim was to compare the quantifiable output of 1 of the LFAs to ID antibody titers. Sample: Serum banked from 73 client-owned dogs diagnosed with pulmonary or disseminated coccidioidomycosis. Methods: ID was used to determine antibody presence and titer against a coccidioidal antigen preparation. All sera were subsequently tested on an LFA based on recombinant chitinase 1 (CTS1) and the commercially available sōna LFA. LFA results were analyzed and compared to ID IgG results and clinical diagnosis. Results: All assays showed similar sensitivities in detecting anti-coccidioidal antibodies (83.6% to 89.0%). When compared with ID IgG, the CTS1 LFA had a positive percent agreement of 100%, while the sōna LFA had a positive percent agreement of 91.4%. Since the CTS1 LFA is semiquantitative, we were able to compare test line densities with ID titers and found a strong correlation between the 2 assays (Spearman ρ = 0.82). Clinical Relevance: This is the first side-by-side evaluation of a commercially available LFA (sōna) and a newer more rapid anti-CTS1 antibody LFA using serum from dogs with coccidioidomycosis. Both LFAs tested have similar sensitivity to ID IgG results. The CTS1 LFA can be read after 10 minutes and is semiquantitative, while the sōna LFA is read after 30 minutes, and the results are subject to interpretation. Accurate and fast detection of anti-coccidioidal antibodies allows clinicians to initiate appropriate treatment without diagnostic delay.
Databáze:	MEDLINE
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