Glutamine-mediated Modulation of XO/uric acid/NF-kB Signaling Pathway Ameliorates Intestinal I/R-induced Bacterial Translocation and Cardiorenal Inflammatory Injury.

Autor: Hamed MA; Department of Medical Laboratory Science, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria.; The Brainwill Laboratory, Osogbo, Osun State, Nigeria.; Reproductive Biology and Toxicology Research Laboratory, Oasis of Grace Hospital, Osogbo, Osun State, Nigeria., Adegboyega OO; Department of Medical Microbiology and Parasitology, Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Nigeria., Ojo OI; Department of Medical Microbiology and Parasitology, Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Nigeria., Akhigbe TM; Reproductive Biology and Toxicology Research Laboratory, Oasis of Grace Hospital, Osogbo, Osun State, Nigeria.; Breeding and Genetics Unit, Department of Agronomy, Osun State University, Ejigbo Campus, Osun State, Nigeria., Fajuyitan FD; The Brainwill Laboratory, Osogbo, Osun State, Nigeria.; Department of Microbiology, Faculty of Sciences, Federal University Oye-Ekiti, Oye-Ekiti, Ekiti State, Nigeria., Adeyemo OC; The Brainwill Laboratory, Osogbo, Osun State, Nigeria.; Department of Microbiology, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, Osun State, Nigeria., Odebunmi TF; The Brainwill Laboratory, Osogbo, Osun State, Nigeria.; Department of Microbiology, Faculty of Sciences, Federal University Oye-Ekiti, Oye-Ekiti, Ekiti State, Nigeria., Adeniyi OS; The Brainwill Laboratory, Osogbo, Osun State, Nigeria.; Department of Microbiology, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, Osun State, Nigeria., Omole IA; The Brainwill Laboratory, Osogbo, Osun State, Nigeria.; Reproductive Biology and Toxicology Research Laboratory, Oasis of Grace Hospital, Osogbo, Osun State, Nigeria.; Department of Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Akhigbe RE; Reproductive Biology and Toxicology Research Laboratory, Oasis of Grace Hospital, Osogbo, Osun State, Nigeria. reakhigbe@lautech.edu.ng.; Department of Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria. reakhigbe@lautech.edu.ng.
Jazyk: angličtina
Zdroj: Cell biochemistry and biophysics [Cell Biochem Biophys] 2024 Jun; Vol. 82 (2), pp. 1007-1018. Date of Electronic Publication: 2024 Mar 26.
DOI: 10.1007/s12013-024-01252-6
Abstrakt: This study explored the effect of intestinal ischaemia/reperfusion (I/R) on cardiorenal tissues. The involvement of xanthine oxidase/uric acid/NF-kB signaling in intestinal I/R was also investigated. In addition, the possible protective effect of glutamine was also evaluated. Twenty-four male Wistar rats were acclimatized and then randomly assigned to four groups (n = 6); sham-operated, glutamine-treated rats (GLUT), I/R, and I/R + GLUT. The sham-operated rats were sham-operated and received 0.5 mL of distilled water, GLUT rats were sham-operated and had 1 g/kg b.w. of glutamine, I/R animals had an intestinal I/R procedure and received 0.5 mL of distilled water, and the I/R + GLUT rats had an intestinal I/R procedure and also received 1 g/kg b.w. of glutamine. Treatments were daily and per os. Glutamine attenuated intestinal I/R-induced rise in intestinal and cardiorenal activities of creatinine kinase and lactate dehydrogenase and lactate level. More so, glutamine alleviated I/R-induced rise in malondialdehyde, xanthine oxidase, uric acid, myeloperoxidase, NF-kB, TNF-α, IL-1β, caspase 3 activity, and DNA fragmentation. Furthermore, glutamine suppressed I/R-induced decline in GSH levels and SOD and catalase activities. Moreover, glutamine improved intestinal, cardiac, and renal histology in animals subjected to intestinal I/R.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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