Plant regeneration capacity in seeds of three species of Miconia (Melastomataceae) may be related to endogenous polyamine profiles.

Autor: Ziemmer JK; Programa de Pós-Graduação em Biologia Vegetal, Campinas, Universidade Estadual de Campinas, São Paulo, 13083-862, Brazil. juliana.ziemmer@gmail.com., Dos Reis de Oliveira T; Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, Rio de Janeiro, 28013-602, Brazil., Santa-Catarina C; Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, Rio de Janeiro, 28013-602, Brazil., do Nascimento Vieira L; Departamento de Botânica, Universidade Federal do Paraná, Curitiba, Paraná, 81531-970, Brazil., Goldenberg R; Departamento de Botânica, Universidade Federal do Paraná, Curitiba, Paraná, 81531-970, Brazil., Pacheco de Freitas Fraga H; Departamento de Botânica, Universidade Federal do Paraná, Curitiba, Paraná, 81531-970, Brazil.
Jazyk: angličtina
Zdroj: Protoplasma [Protoplasma] 2024 Sep; Vol. 261 (5), pp. 937-950. Date of Electronic Publication: 2024 Mar 26.
DOI: 10.1007/s00709-024-01945-y
Abstrakt: In plant tissue culture, differences in endogenous levels of species-specific plant growth regulators (PGRs) may explain differences in regenerative capacity. In the case of polyamines (PAs), their dynamics and distribution may vary between species, genotypes, tissues, and developmental pathways, such as sexual reproduction and apomixis. In this study, for the first time, we aimed to assess the impact of varying endogenous PAs levels in seeds from distinct reproductive modes in Miconia spp. (Melastomataceae), on their in vitro regenerative capacity. We quantified the free PAs endogenous content in seeds of Miconia australis (obligate apomictic), Miconia hyemalis (facultative apomictic), and Miconia sellowiana (sexual) and evaluated their in vitro regenerative potential in WPM culture medium supplemented with a combination of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP). The morphogenic responses were characterized by light microscopy and scanning electron microscopy and discussed regarding the endogenous PAs profiles found. Seeds of M. sellowiana presented approximately eight times more putrescine than M. australis, which was associated with a higher percentage of regenerated calluses (76.67%) than M. australis (5.56%). On the other hand, spermine levels were significantly higher in M. australis. Spermine is indicated as an inhibitor of auxin-carrying gene expression, which may have contributed to its lower regenerative capacity under the tested conditions. These findings provide important insights into in vitro morphogenesis mechanisms in Miconia and highlight the significance of endogenous PA levels in plant regeneration. These discoveries can potentially optimize future regeneration protocols in Miconia, a plant group still underexplored in this area.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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