Divergent roles of IREG/Ferroportin transporters from the nickel hyperaccumulator Leucocroton havanensis.

Autor: González DA; Jardín Botánico Nacional, Universidad de La Habana, La Habana, Cuba.; Universidad Agraria de La Habana, Facultad de Agronomía, San José de las Lajas, Mayabeque, Cuba., de la Torre VSG; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France., Fernández RR; Universidad Agraria de La Habana, Facultad de Agronomía, San José de las Lajas, Mayabeque, Cuba., Barreau L; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France., Merlot S; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France.; Laboratoire de Recherche en Sciences Végétales (LRSV), UMR5546 CNRS/UPS/INPT, France.
Jazyk: angličtina
Zdroj: Physiologia plantarum [Physiol Plant] 2024 Mar-Apr; Vol. 176 (2), pp. e14261.
DOI: 10.1111/ppl.14261
Abstrakt: In response to our ever-increasing demand for metals, phytotechnologies are being developed to limit the environmental impact of conventional metal mining. However, the development of these technologies, which rely on plant species able to tolerate and accumulate metals, is partly limited by our lack of knowledge of the underlying molecular mechanisms. In this work, we aimed to better understand the role of metal transporters of the IRON REGULATED 1/FERROPORTIN (IREG/FPN) family from the nickel hyperaccumulator Leucocroton havanensis from the Euphorbiaceae family. Using transcriptomic data, we identified two homologous genes, LhavIREG1 and LhavIREG2, encoding divalent metal transporters of the IREG/FPN family. Both genes are expressed at similar levels in shoots, but LhavIREG1 shows higher expression in roots. The heterologous expression of these transporters in A. thaliana revealed that LhavIREG1 is localized to the plasma membrane, whereas LhavIREG2 is located on the vacuole. In addition, the expression of each gene induced a significant increase in nickel tolerance. Taken together, our data suggest that LhavIREG2 is involved in nickel sequestration in vacuoles of leaf cells, whereas LhavIREG1 is mainly involved in nickel translocation from roots to shoots, but could also be involved in metal sequestration in cell walls. Our results suggest that paralogous IREG/FPN transporters may play complementary roles in nickel hyperaccumulation in plants.
(© 2024 The Authors. Physiologia Plantarum published by John Wiley & Sons Ltd on behalf of Scandinavian Plant Physiology Society.)
Databáze: MEDLINE
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