An improved mouse model of sepsis based on intraperitoneal injections of the enriched culture of cecum slurry.

Autor: Atre R; Department of Biosciences and Biomedical Engineering (BSBE), Indian Institute of Technology Indore (IITI), Indore, MP 453552, India., Sharma R; Department of Biosciences and Biomedical Engineering (BSBE), Indian Institute of Technology Indore (IITI), Indore, MP 453552, India., Obukhov AG; Department of Anatomy, Cell Biology & Physiology, Indiana University School of Medicine, Indianapolis, IN 46202, USA; Stark Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis, IN 46202, USA., Saqib U; School of Life Sciences, Devi Ahilya Vishwavidyalaya, Vigyan Bhawan, Indore, MP 452 001, India., Umar S; Department of Oral Biology, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USA., Darwhekar GN; Acropolis Institute of Pharmaceutical Education and Research (AIPER), Indore, MP 453771, India., Baig MS; Department of Biosciences and Biomedical Engineering (BSBE), Indian Institute of Technology Indore (IITI), Indore, MP 453552, India. Electronic address: msb.iit@iiti.ac.in.
Jazyk: angličtina
Zdroj: Life sciences [Life Sci] 2024 May 15; Vol. 345, pp. 122584. Date of Electronic Publication: 2024 Mar 26.
DOI: 10.1016/j.lfs.2024.122584
Abstrakt: Aim: Sepsis is a life-threatening clinical syndrome comprising multiorgan dysfunctions caused by a disproportionate body immune response. There are several animal sepsis models which are based on cecum ligation, cecal puncture, and cecum slurry injection. The major limitation of all current sepsis models is the high variability owing to the variable degree of ligation, puncture and inconsistent microbial composition used for sepsis initiation. The primary objective of this work is to demonstrate the feasibility of a standardized method for sepsis development.
Materials and Methods: The cecal slurry bacterial culture was developed and preserved in glycerol stocks. Antibiotics aztreonam and vancomycin were used for generating several defined, enriched cecal slurry bacterial cultures. Mice survival was assessed until 48 hrs post injection, and the tissue samples were collected after 10 hrs from sepsis initiation.
Key Findings: The results indicate that increasing polymicrobial load resulted in lower survival rates and was associated with the higher number of infiltrating immune cells and necrosis. H&E (haematoxylin & eosin) staining & serum markers revealed that septic mice exhibited increased inflammation and significant damage to the liver and kidneys. The defined Gram-negative and Gram-positive specific cecal slurry bacterial cultures were developed and their efficiency in inducing sepsis was characterized.
Significance: Enriched cecal slurry bacterial cultures can be stored in glycerol stocks at -80 °C. This has an ethical advantage of avoiding unnecessary animal euthanasia for each experiment and provides a standardization capability of sepsis development.
Competing Interests: Declaration of competing interest No competing interests declared.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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