Quaternization of cassava starch and determination of antimicrobial activity against bacteria and coronavirus.

Autor: Ribeiro de Carvalho G; Department of Bioprocesses and Materials Engineering, School of Chemical Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil. Electronic address: gr.carvalho@yahoo.com.br., Kudaka AM; Department of Bioprocesses and Materials Engineering, School of Chemical Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil., Fares Sampar J; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil., Alvares LE; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil., Delarmelina C; Chemical, Biological and Agricultural Pluridisciplinary Research Center, University of Campinas (UNICAMP), Paulínia, SP, Brazil., Duarte MCT; Chemical, Biological and Agricultural Pluridisciplinary Research Center, University of Campinas (UNICAMP), Paulínia, SP, Brazil., Lona LMF; Department of Bioprocesses and Materials Engineering, School of Chemical Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil. Electronic address: lona@unicamp.br.
Jazyk: angličtina
Zdroj: Carbohydrate research [Carbohydr Res] 2024 Apr; Vol. 538, pp. 109098. Date of Electronic Publication: 2024 Mar 21.
DOI: 10.1016/j.carres.2024.109098
Abstrakt: This study describes the novel development of quaternized cassava starch (Q-CS) with antimicrobial and antiviral properties, particularly effective against the MHV-3 coronavirus. The preparation of Q-CS involved the reaction of cassava starch (CS) with glycidyltrimethylammonium chloride (GTMAC) in an alkaline solution. Q-CS physicochemical properties were determined by FTIR, NMR, elemental analysis, zeta potential, TGA, and moisture sorption. FTIR and NMR spectra confirmed the introduction of cationic groups in the CS structure. The elemental analysis revealed a degree of substitution (DS) of 0.552 of the cationic reagent on the hydroxyl groups of CS. Furthermore, Q-CS exhibited a positive zeta potential value (+28.6 ± 0.60 mV) attributed to the high positive charge density shown by the quaternary ammonium groups. Q-CS demonstrated lower thermal stability and higher moisture sorption compared to CS. The antimicrobial activity of Q-CS was confirmed against Escherichia coli (MIC = 0.156 mg mL -1 ) and Staphylococcus aureus (MIC = 0.312 mg mL -1 ), along with a remarkable ability to inactivate 99% of MHV-3 coronavirus after only 1 min of direct contact. Additionally, Q-CS showed high cell viability (close to 100%) and minimal cytotoxicity effects, guaranteeing its safe use. Therefore, these findings indicate the potential use of Q-CS as a raw material for antiseptic biomaterials.
Competing Interests: Declaration of competing interest The authors declare no conflicts of interest.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: