Protocols for Building and Producing High Diversity Peptide Phage Display Libraries.
| Autor: | Giordano RJ; Biochemistry Department, Institute of Chemistry, University of Sao Paulo, Sao Paulo, Brazil. giordano@iq.usp.br., Alecrim LC; Biochemistry Department, Institute of Chemistry, University of Sao Paulo, Sao Paulo, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2793, pp. 3-19. |
| DOI: | 10.1007/978-1-0716-3798-2_1 |
| Abstrakt: | Phage display is an important technology to study protein-protein interaction and protein evolution, with applications in basic science and applied biotechnology, such as drug discovery and the development of targeted therapies. However, in order to be successful during a phage display screening, it is paramount to have good phage libraries. Here, we described detailed procedures to generate peptide phage display libraries with high diversity and billions of transformants. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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