New long noncoding RNA biomarkers and ceRNA networks on miR-616-3p in colorectal cancer: Bioinformatics-based study.

Autor:	Abdolvand M; Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.; Medical Genetics Research Center of Genome, Isfahan University of Medical Sciences, Isfahan, Iran.; Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran., Chermahini ZM; Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran., Bahaloo S; Department of Biology, Faculty of Sciences, Yazd University, Yazd, Iran., Emami MH; Poursina Hakim Digestive Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran., Fahim A; Poursina Hakim Digestive Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran., Rahimi H; Poursina Hakim Digestive Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran., Amjadi E; Poursina Hakim Digestive Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran., Maghool F; Poursina Hakim Digestive Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran., Rohani F; Faculty of Veterinary Medicine of Shahrekord, Shahrekord, Iran., Dadkhah M; Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran., Farhadian N; Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran., Vatandoust N; Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran., Abdolvand S; Department of Genetics, Faculty of Sciences, Islamic Azad University, Shahrekord, Iran., Darehsari MR; Department of Genetics, Faculty of Sciences, Islamic Azad University, Shahrekord, Iran., Chehelgerdi M; Department of Genetics, Faculty of Sciences, Islamic Azad University, Shahrekord, Iran., Beni FA; Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.; Medical Genetics Research Center of Genome, Isfahan University of Medical Sciences, Isfahan, Iran.; Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran., Khodadoostan M; Department of Gastroenterology and Hepatology, AlZahra Hospital, Isfahan University of Medical Sciences, Isfahan, Iran., Hemati S; Department of Radiooncology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran., Salehi M; Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.; Medical Genetics Research Center of Genome, Isfahan University of Medical Sciences, Isfahan, Iran.; Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Jazyk:	angličtina
Zdroj:	Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences [J Res Med Sci] 2024 Feb 23; Vol. 29, pp. 10. Date of Electronic Publication: 2024 Feb 23 (Print Publication: 2024).
DOI:	10.4103/jrms.jrms_786_22
Abstrakt:	Background: Cancer development is aided by the role of long noncoding RNAs (lncRNAs) that act as competing endogenous RNAs (ceRNAs) absorbing microRNAs (miRNAs). We aimed to discover a novel regulatory axis in colorectal cancer (CRC) and potential biomarkers based on miR-616-3p. Materials and Methods: The gene expression omnibus database was mined for differentially expressed lncRNAs (DELs) and mRNAs. LncRNAs and mRNAs were predicted using the RegRNA and TargetScan databases. A combination of the ciBioPortal and Ensemble databases was used to locate the mRNAs. Cytoscape 3.7.1-built CeRNA networks. A quantitative real-time polymerase chain reaction (qRT-PCR) was utilized to confirm the expression levels of these RNA molecules. Statistical analyses were implemented by GraphPad Prism 9. Results: qRT-PCR showed (Linc01282, lnc-MYADM-1:1, and Zinc Finger Protein 347 [ZNF347]) were overexpressed whereas, (salt-inducible kinases 1 [SIK1], and miR-616-3p) were down regulated. Conclusion: These results identify unique, unreported lncRNAs as CRC prognostic biomarkers, as well as prospective mRNAs as new treatment targets and predictive biomarkers for CRC. In addition, our study uncovered unexplored ceRNA networks that should be studied further in CRC. Competing Interests: There are no conflicts of interest. (Copyright: © 2024 Journal of Research in Medical Sciences.)
Databáze:	MEDLINE
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