Chlorpyrifos impairs sperm parameters and number of Sertoli and Leydig cells in rats after exposure during the peripubertal period.

Autor: da Costa IR; Department of General Pathology, Biological Sciences Center, State University of Londrina - UEL, Londrina, Paraná, Brazil; Department of General Biology, Biological Sciences Center, State University of Londrina - UEL, Londrina, Paraná, Brazil., Quadreli DH; Department of General Biology, Biological Sciences Center, State University of Londrina - UEL, Londrina, Paraná, Brazil., da Silva LMM; Department of Physiological Sciences, Biological Sciences Center, State University of Londrina - UEL, Londrina, Paraná, Brazil., de Andrade FG; Department of Histology, Biological Sciences Center, State University of Londrina - UEL, Londrina, Paraná, Brazil., Fernandes GSA; Department of General Biology, Biological Sciences Center, State University of Londrina - UEL, Londrina, Paraná, Brazil. Electronic address: glaura@uel.br.
Jazyk: angličtina
Zdroj: Toxicology [Toxicology] 2024 May; Vol. 504, pp. 153789. Date of Electronic Publication: 2024 Mar 23.
DOI: 10.1016/j.tox.2024.153789
Abstrakt: Chlorpyrifos is an organophosphate insecticide used to control pests in crops. Thus, humans are constantly exposed through ingestion of contaminated food or water, inhalation of contaminated air, and through the skin. The juvenile and peripubertal periods comprise a window of development of the reproductive system, sensitive to toxic agents. Considering the scarcity of data on exposure to the insecticide during these periods, the aim of this study was to evaluate the effects of chlorpyrifos on the testis during the juvenile and peripubertal periods. Thirty Wistar rats with an initial age of 25 days were distributed into 3 groups: control, which received corn oil (vehicle); CPS5, which received 5 mg/Kg b.w. of chlorpyrifos; and CPS15, which received 15 mg/Kg b.w. of chlorpyrifos. The groups were treated via gavage daily for 40 days and on the 41st experimental day, the animals were anesthetized and submitted to euthanasia to collect the organs. Blood was collected to obtain plasma and testosterone measurement. The testicles were removed, weighed and used for sperm count analyses, histopathological and morphometric analyzes and for oxidative stress analyses. Spermatozoa from the vas deferens were collected for analyzes of sperm morphology and acrosome integrity. The results showed that the two concentrations of chlorpyrifos caused a decrease in the number of Leydig and Sertoli cells and germ cells and increased the number of morphologically abnormal sperm and sperm with acrosomal damage. Furthermore, a decrease in lipid peroxidation was observed in the CPS5 and CPS15 groups, and a decrease in glutathione-S-transferase activity in the CPS5 group. We conclude that exposure to chlorpyrifos harms the daily production of sperm, as well as their quality, in addition to causing an imbalance in the oxidoreductive balance of the testicle.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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