Redox-activity and in vitro effects of regional atmospheric aerosol pollution: Seasonal differences and correlation between oxidative potential and in vitro toxicity of PM 1 .

Autor: Melzi G; Department of Pharmacological and Biomolecular Science (DiSFeB) 'Rodolfo Paoletti', Università degli Studi di Milano, Via Balzaretti 9, 20133 Milan, Italy. Electronic address: gloria.melzi@unimi.it., Massimi L; Department of Environmental Biology, Sapienza University of Rome, P.le Aldo Moro, 5, 00185 Rome, Italy; Institute of Atmospheric Pollution Research, National Research Council, Via Salaria, Km 29,300, Monterotondo St., 00015 Rome, Italy., Frezzini MA; Department of Environmental Biology, Sapienza University of Rome, P.le Aldo Moro, 5, 00185 Rome, Italy; ARPA Lazio, Regional Environmental Protection Agency, Via Boncompagni 101, 00187 Rome, Italy., Iulini M; Department of Pharmacological and Biomolecular Science (DiSFeB) 'Rodolfo Paoletti', Università degli Studi di Milano, Via Balzaretti 9, 20133 Milan, Italy., Tarallo N; Department of Pharmacological and Biomolecular Science (DiSFeB) 'Rodolfo Paoletti', Università degli Studi di Milano, Via Balzaretti 9, 20133 Milan, Italy., Rinaldi M; Institute of Atmospheric Sciences and Climate, National Research Council, 40129 Bologna, Italy; National Biodiversity Future Center, NBFC, 90133 Palermo, Italy., Paglione M; Institute of Atmospheric Sciences and Climate, National Research Council, 40129 Bologna, Italy; National Biodiversity Future Center, NBFC, 90133 Palermo, Italy., Nozza E; Department of Pharmacological and Biomolecular Science (DiSFeB) 'Rodolfo Paoletti', Università degli Studi di Milano, Via Balzaretti 9, 20133 Milan, Italy; Experimental Medicine, Università degli Studi di Milano, Via L. Vanvitelli 32, 20129 Milan, Italy; Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Via della Commenda 19, 20122 Milan, Italy., Crova F; Department of Physics, Università degli Studi di Milano & INFN-Milano, Via Celoria 16, 20133 Milan, Italy., Valentini S; Department of Physics, Università degli Studi di Milano & INFN-Milano, Via Celoria 16, 20133 Milan, Italy., Valli G; Department of Physics, Università degli Studi di Milano & INFN-Milano, Via Celoria 16, 20133 Milan, Italy., Costabile F; National Biodiversity Future Center, NBFC, 90133 Palermo, Italy; Institute of Atmospheric Sciences and Climate, National Research Council, Via Fosso del Cavaliere 100, Rome, Italy., Canepari S; Department of Environmental Biology, Sapienza University of Rome, P.le Aldo Moro, 5, 00185 Rome, Italy; Institute of Atmospheric Pollution Research, National Research Council, Via Salaria, Km 29,300, Monterotondo St., 00015 Rome, Italy., Decesari S; Institute of Atmospheric Sciences and Climate, National Research Council, 40129 Bologna, Italy; National Biodiversity Future Center, NBFC, 90133 Palermo, Italy., Vecchi R; Department of Physics, Università degli Studi di Milano & INFN-Milano, Via Celoria 16, 20133 Milan, Italy., Marinovich M; Department of Pharmacological and Biomolecular Science (DiSFeB) 'Rodolfo Paoletti', Università degli Studi di Milano, Via Balzaretti 9, 20133 Milan, Italy., Corsini E; Department of Pharmacological and Biomolecular Science (DiSFeB) 'Rodolfo Paoletti', Università degli Studi di Milano, Via Balzaretti 9, 20133 Milan, Italy.
Jazyk: angličtina
Zdroj: Toxicology and applied pharmacology [Toxicol Appl Pharmacol] 2024 Apr; Vol. 485, pp. 116913. Date of Electronic Publication: 2024 Mar 24.
DOI: 10.1016/j.taap.2024.116913
Abstrakt: Particulate Matter (PM) is a complex and heterogeneous mixture of atmospheric particles recognized as a threat to human health. Oxidative Potential (OP) measurement is a promising and integrative method for estimating PM-induced health impacts since it is recognized as more closely associated with adverse health effects than ordinarily used PM mass concentrations. OP measurements could be introduced in the air quality monitoring, along with the parameters currently evaluated. PM deposition in the lungs induces oxidative stress, inflammation, and DNA damage. The study aimed to compare the OP measurements with toxicological effects on BEAS-2B and THP-1 cells of winter and summer PM 1 collected in the Po Valley (Italy) during 2021. PM 1 was extracted in deionized water by mechanical agitation and tested for OP and, in parallel, used to treat cells. Cytotoxicity, genotoxicity, oxidative stress, and inflammatory responses were assessed by MTT test, DCFH-DA assay, micronucleus, γ-H2AX, comet assay modified with endonucleases, ELISA, and Real-Time PCR. The evaluation of OP was performed by applying three different assays: dithiothreitol (OP DTT ), ascorbic acid (OP AA ), and 2',7'-dichlorofluorescein (OP DCFH ), in addition, the reducing potential was also analysed (RP DPPH ). Seasonal differences were detected in all the parameters investigated. The amount of DNA damage detected with the Comet assay and ROS formation highlights the presence of oxidative damage both in winter and in summer samples, while DNA damage (micronucleus) and genes regulation were mainly detected in winter samples. A positive correlation with OP DCFH (Spearman's analysis, p < 0.05) was detected for IL-8 secretion and γ-H2AX. These results provide a biological support to the implementation in air quality monitoring of OP measurements as a useful proxy to estimate PM-induced cellular toxicological responses. In addition, these results provide new insights for the assessment of the ability of secondary aerosol in the background atmosphere to induce oxidative stress and health effects.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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