PGI1-mediated vascular oxidative pentose phosphate pathway modulates photosynthesis via long-distance cytokinin signaling.

Autor: Sánchez-López ÁM; Instituto de Agrobiotecnología (IdAB), CSIC-Gobierno de Navarra, Iruñako etorbidea 123, 31192, Mutiloabeti, Nafarroa, Spain., Bahaji A; Instituto de Agrobiotecnología (IdAB), CSIC-Gobierno de Navarra, Iruñako etorbidea 123, 31192, Mutiloabeti, Nafarroa, Spain. Electronic address: abdellatif.bahaji@csic.es., Gámez-Arcas S; Instituto de Agrobiotecnología (IdAB), CSIC-Gobierno de Navarra, Iruñako etorbidea 123, 31192, Mutiloabeti, Nafarroa, Spain; Instituto de Bioquímica Vegetal y Fotosíntesis (IBVF), CSIC-Universidad de Sevilla, 41092, Sevilla, Spain., De Diego N; Czech Advanced Technology and Research Institute, Palacky University, Šlechtitelů 27, 78371, Olomouc, Czech Republic., Vrobel O; Czech Advanced Technology and Research Institute, Palacky University, Šlechtitelů 27, 78371, Olomouc, Czech Republic., Tarkowski P; Czech Advanced Technology and Research Institute, Palacky University, Šlechtitelů 27, 78371, Olomouc, Czech Republic., Baroja-Fernández E; Instituto de Agrobiotecnología (IdAB), CSIC-Gobierno de Navarra, Iruñako etorbidea 123, 31192, Mutiloabeti, Nafarroa, Spain., Muñoz FJ; Instituto de Agrobiotecnología (IdAB), CSIC-Gobierno de Navarra, Iruñako etorbidea 123, 31192, Mutiloabeti, Nafarroa, Spain., Almagro G; Instituto de Agrobiotecnología (IdAB), CSIC-Gobierno de Navarra, Iruñako etorbidea 123, 31192, Mutiloabeti, Nafarroa, Spain., Seguí-Simarro JM; COMAV - Universitat Politècnica de València, Valencia, Spain., Tabernero-Mendoza M; COMAV - Universitat Politècnica de València, Valencia, Spain., López-Serrano L; Institute for Mediterranean and Subtropical Horticulture 'La Mayora' (IHSM), CSIC-UMA, Campus de Teatinos, Avda. Louis Pasteur, 49, 29010, Málaga, Spain., Morcillo RJL; Institute for Mediterranean and Subtropical Horticulture 'La Mayora' (IHSM), CSIC-UMA, Campus de Teatinos, Avda. Louis Pasteur, 49, 29010, Málaga, Spain., Pozueta-Romero J; Instituto de Agrobiotecnología (IdAB), CSIC-Gobierno de Navarra, Iruñako etorbidea 123, 31192, Mutiloabeti, Nafarroa, Spain; Institute for Mediterranean and Subtropical Horticulture 'La Mayora' (IHSM), CSIC-UMA, Campus de Teatinos, Avda. Louis Pasteur, 49, 29010, Málaga, Spain. Electronic address: javier.pozueta@csic.es.
Jazyk: angličtina
Zdroj: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2024 Apr; Vol. 209, pp. 108520. Date of Electronic Publication: 2024 Mar 12.
DOI: 10.1016/j.plaphy.2024.108520
Abstrakt: In Arabidopsis, the plastidial isoform of phosphoglucose isomerase, PGI1, mediates growth and photosynthesis, likely due to its involvement in the vascular production of cytokinins (CK). To examine this hypothesis, we characterized pgi1-2 knockout plants impaired in PGI1 and pgi1-2 plants specifically expressing PGI1 in root tips and vascular tissues. Moreover, to investigate whether the phenotype of pgi1-2 plants is due to impairments in the plastidial oxidative pentose phosphate pathway (OPPP) or the glycolytic pathway, we characterized pgl3-1 plants with reduced OPPP and pfk4pfk5 knockout plants impaired in plastidial glycolysis. Compared with wild-type (WT) leaves, pgi1-2 leaves exhibited weaker expression of photosynthesis- and 2-C-methyl-D-erythritol 4-P (MEP) pathway-related proteins, and stronger expression of oxidative stress protection-related enzymes. Consistently, pgi1-2 leaves accumulated lower levels of chlorophyll, and higher levels of tocopherols, flavonols and anthocyanins than the WT. Vascular- and root tip-specific PGI1 expression countered the reduced photosynthesis, low MEP pathway-derived CK content, dwarf phenotype and the metabolic characteristics of pgi1-2 plants, reverting them to WT-like levels. Moreover, pgl3-1, but not pfk4pfk5 plants phenocopied pgi1-2. Histochemical analyses of plants expressing GUS under the control of promoter regions of genes encoding plastidial OPPP enzymes exhibited strong GUS activity in root tips and vascular tissues. Overall, our findings show that root tip and vascular PGI1-mediated plastidial OPPP activity affects photosynthesis and growth through mechanisms involving long-distance modulation of the leaf proteome by MEP pathway-derived CKs.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
Databáze: MEDLINE
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