Microbiome changes under enteral deprivation are dynamic and dependent on intestinal location.

Autor: Bar-Yoseph H; Gastroenterology Department, Rambam Health Care Campus, Haifa, Israel.; Rappaport Faculty of Medicine, Technion - Israel Institute of Technology, Haifa, Israel., Metcalfe-Roach A; Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.; Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada., Cirstea M; Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.; Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada., Finlay BB; Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.; Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada.
Jazyk: angličtina
Zdroj: JPEN. Journal of parenteral and enteral nutrition [JPEN J Parenter Enteral Nutr] 2024 May; Vol. 48 (4), pp. 502-511. Date of Electronic Publication: 2024 Mar 24.
DOI: 10.1002/jpen.2624
Abstrakt: Background: The microbiome has a pivotal role in intestinal health, and nutrition has a major role shaping its structure. Enteral deprivation, in which no oral/enteral nutrition is administered, is common in hospitalized/gastrointestinal patients. The dynamics that enteral deprivation exerts on the microbial community, specifically in the small intestine, are not well understood.
Methods: Enteral deprivation was modeled with exclusive parenteral nutrition (EPN) mice. Mice were allocated to receive either EPN or saline and chow (control) and euthanized after 0, 2, 4, or 6 days. DNA was extracted from jejunum, ileum, and colon content. 16S sequencing was used to compare changes in microbial communities between groups. Functional pathways were predicted using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States.
Results: EPN-treated mice showed community changes throughout the intestine. Beta diversity in colon showed clear separation between the groups (Bray-Curtis, P < 0.001). Time-dependent dynamics were seen in ileal but not jejunal samples. Alpha diversity was lower in the colon of EPN mice compared with control/baseline mice (Chao1, P < 0.01) but not in ileum/jejunum. Progressive loss of single-taxon domination was seen, most notably in the small intestine. This was accompanied by increases/decreases in specific taxa. A clear separation was seen in the functional capacity of the community between fed and enterally deprived mice at the ileum and colon, which was observed early on.
Conclusions: Enteral deprivation disturbs the microbial community in a spatial and dynamic manner. There should be further focus on studying the effect of these changes on the host.
(© 2024 The Authors. Journal of Parenteral and Enteral Nutrition published by Wiley Periodicals LLC on behalf of American Society for Parenteral and Enteral Nutrition.)
Databáze: MEDLINE
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