Single-molecule observation of G-quadruplex and R-loop formation induced by transcription.

Autor:	Hwang J; Program in Cellular and Molecular Medicine, Boston Children's Hospital and Harvard Medical School, Boston, MA, United States., Palmer B; Program in Cellular Molecular Developmental Biology and Biophysics, Johns Hopkins University, Baltimore, MD, United States., Myong S; Program in Cellular and Molecular Medicine, Boston Children's Hospital and Harvard Medical School, Boston, MA, United States; Program in Cellular Molecular Developmental Biology and Biophysics, Johns Hopkins University, Baltimore, MD, United States. Electronic address: sua.myong@childrens.harvard.edu.
Jazyk:	angličtina
Zdroj:	Methods in enzymology [Methods Enzymol] 2024; Vol. 695, pp. 71-88. Date of Electronic Publication: 2024 Jan 16.
DOI:	10.1016/bs.mie.2024.01.001
Abstrakt:	Potential G-quadruplex forming sequences (PQS) are enriched in cancer-related genes and immunoglobulin class-switch recombination. They are prevalent in the 5'UTR of transcriptionally active genes, thereby contributing to the regulation of gene expression. We and others previously demonstrated that the PQS located in the non-template strand leads to an R-loop formation followed by a G-quadruplex (G4) formation during transcription. These structural changes increase mRNA production. Here, we present how single-molecule technique was used to observe cotranscriptional G4 and R-loop formation and to examine the impact on transcription, particularly for the initiation and elongation stages. (Copyright © 2024. Published by Elsevier Inc.)
Databáze:	MEDLINE
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