Thioredoxins o1 and h2 jointly adjust mitochondrial dihydrolipoamide dehydrogenase-dependent pathways towards changing environments.

Autor: Timm S; Plant Physiology Department, University of Rostock, Rostock, Germany., Klaas N; Plant Physiology Department, University of Rostock, Rostock, Germany., Niemann J; Plant Physiology Department, University of Rostock, Rostock, Germany., Jahnke K; Plant Physiology Department, University of Rostock, Rostock, Germany., Alseekh S; Max Planck Institute of Molecular Plant Physiology, Golm, Germany., Zhang Y; Max Planck Institute of Molecular Plant Physiology, Golm, Germany.; Center of Plant System Biology and Biotechnology, Plovdiv, Bulgaria., Souza PVL; LabPlant, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Brazil., Hou LY; Department Biology I, Ludwig-Maximilians-University Munich, Planegg-Martinsried, Germany.; Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan., Cosse M; Department of Plant Cell Biology, Botanical Institute, Christian-Albrechts University Kiel, Kiel, Germany., Selinski J; Department of Plant Cell Biology, Botanical Institute, Christian-Albrechts University Kiel, Kiel, Germany., Geigenberger P; Department Biology I, Ludwig-Maximilians-University Munich, Planegg-Martinsried, Germany., Daloso DM; LabPlant, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Brazil., Fernie AR; Max Planck Institute of Molecular Plant Physiology, Golm, Germany.; Center of Plant System Biology and Biotechnology, Plovdiv, Bulgaria., Hagemann M; Plant Physiology Department, University of Rostock, Rostock, Germany.
Jazyk: angličtina
Zdroj: Plant, cell & environment [Plant Cell Environ] 2024 Jul; Vol. 47 (7), pp. 2542-2560. Date of Electronic Publication: 2024 Mar 22.
DOI: 10.1111/pce.14899
Abstrakt: Thioredoxins (TRXs) are central to redox regulation, modulating enzyme activities to adapt metabolism to environmental changes. Previous research emphasized mitochondrial and microsomal TRX o1 and h2 influence on mitochondrial metabolism, including photorespiration and the tricarboxylic acid (TCA) cycle. Our study aimed to compare TRX-based regulation circuits towards environmental cues mainly affecting photorespiration. Metabolite snapshots, phenotypes and CO 2 assimilation were compared among single and multiple TRX mutants in the wild-type and the glycine decarboxylase T-protein knockdown (gldt1) background. Our analyses provided evidence for additive negative effects of combined TRX o1 and h2 deficiency on growth and photosynthesis. Especially metabolite accumulation patterns suggest a shared regulation mechanism mainly on mitochondrial dihydrolipoamide dehydrogenase (mtLPD1)-dependent pathways. Quantification of pyridine nucleotides, in conjunction with 13 C-labelling approaches, and biochemical analysis of recombinant mtLPD1 supported this. It also revealed mtLPD1 inhibition by NADH, pointing at an additional measure to fine-tune it's activity. Collectively, we propose that lack of TRX o1 and h2 perturbs the mitochondrial redox state, which impacts on other pathways through shifts in the NADH/NAD + ratio via mtLPD1. This regulation module might represent a node for simultaneous adjustments of photorespiration, the TCA cycle and branched chain amino acid degradation under fluctuating environmental conditions.
(© 2024 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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