Lipid metabolism-related long noncoding RNA RP11-817I4.1 promotes fatty acid synthesis and tumor progression in hepatocellular carcinoma.

Autor:	Wang RY; Second Affiliated Hospital of Kunming Medical University, Kunming 650106, Yunnan Province, China., Yang JL; School of Basic Medical Sciences, Nanjing Medical University, Nanjing 211166, Jiangsu Province, China., Xu N; Second Affiliated Hospital of Kunming Medical University, Kunming 650106, Yunnan Province, China., Xu J; Wuhan Blood Center, Wuhan 430030, Hubei Province, China., Yang SH; Second Affiliated Hospital of Kunming Medical University, Kunming 650106, Yunnan Province, China., Liang DM; Second Affiliated Hospital of Kunming Medical University, Kunming 650106, Yunnan Province, China., Li JZ; Department of Gastrointestinal Surgery, The Third People's Hospital of Hubei Province, Wuhan 430071, Hubei Province, China., Zhu H; Second Affiliated Hospital of Kunming Medical University, Kunming 650106, Yunnan Province, China. zhuhong@kmmu.edu.cn.
Jazyk:	angličtina
Zdroj:	World journal of gastroenterology [World J Gastroenterol] 2024 Feb 28; Vol. 30 (8), pp. 919-942.
DOI:	10.3748/wjg.v30.i8.919
Abstrakt:	Background: Hepatocellular carcinoma (HCC) is one of the most common types of tumors. The influence of lipid metabolism disruption on the development of HCC has been demonstrated in published studies. Aim: To establish an HCC prognostic model for lipid metabolism-related long non-coding RNAs (LMR-lncRNAs) and conduct in-depth research on the specific role of novel LMR-lncRNAs in HCC. Methods: Correlation and differential expression analyses of The Cancer Genome Atlas data were used to identify differentially expressed LMR-lncRNAs. Quantitative real-time polymerase chain reaction analysis was used to evaluate the expression of LMR-lncRNAs. Nile red staining was employed to observe intracellular lipid levels. The interaction between RP11-817I4.1, miR-3120-3p, and ATP citrate lyase (ACLY) was validated through the performance of dual-luciferase reporter gene and RIP assays. Results: Three LMR-lncRNAs (negative regulator of antiviral response, RNA transmembrane and coiled-coil domain family 1 antisense RNA 1, and RP11-817I4.1) were identified as predictive markers for HCC patients and were utilized in the construction of risk models. Additionally, proliferation, migration, and invasion were reduced by RP11-817I4.1 knockdown. An increase in lipid levels in HCC cells was significantly induced by RP11-817I4.1 through the miR-3120-3p/ACLY axis. Conclusion: LMR-lncRNAs have the capacity to predict the clinical characteristics and prognoses of HCC patients, and the discovery of a novel LMR-lncRNAs, RP11-817I4.1, revealed its role in promoting lipid accumulation, thereby accelerating the onset and progression of HCC. Competing Interests: Conflict-of-interest statement: There are no conflicts of interest to disclose in this article. (©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu