Promastigote EPS secretion and haptomonad biofilm formation as evolutionary adaptations of trypanosomatid parasites for colonizing honeybee hosts.

Autor: Carreira de Paula J; Department of Parasitology, Biochemical and Molecular Parasitology Group CTS-183, University of Granada, Granada, Spain.; Institute of Biotechnology, University of Granada, Granada, Spain., García Olmedo P; Department of Parasitology, Biochemical and Molecular Parasitology Group CTS-183, University of Granada, Granada, Spain.; Institute of Biotechnology, University of Granada, Granada, Spain., Gómez-Moracho T; Department of Parasitology, Biochemical and Molecular Parasitology Group CTS-183, University of Granada, Granada, Spain.; Institute of Biotechnology, University of Granada, Granada, Spain., Buendía-Abad M; Laboratorio de Patología Apícola, Centro de Investigación Apícola y Agroambiental (CIAPA), IRIAF - Instituto Regional de Investigación y Desarrollo Agroalimentario y Forestal, Consejería de Agricultura de la Junta de Comunidades de Castilla-La Mancha, Marchamalo, Spain.; Instituto de Recursos Humanos para la Ciencia y la Tecnología, Fundación Parque Científico y Tecnológico de Castilla-La Mancha, 02006, Albacete, Spain., Higes M; Laboratorio de Patología Apícola, Centro de Investigación Apícola y Agroambiental (CIAPA), IRIAF - Instituto Regional de Investigación y Desarrollo Agroalimentario y Forestal, Consejería de Agricultura de la Junta de Comunidades de Castilla-La Mancha, Marchamalo, Spain.; Instituto de Recursos Humanos para la Ciencia y la Tecnología, Fundación Parque Científico y Tecnológico de Castilla-La Mancha, 02006, Albacete, Spain., Martín-Hernández R; Laboratorio de Patología Apícola, Centro de Investigación Apícola y Agroambiental (CIAPA), IRIAF - Instituto Regional de Investigación y Desarrollo Agroalimentario y Forestal, Consejería de Agricultura de la Junta de Comunidades de Castilla-La Mancha, Marchamalo, Spain.; Instituto de Recursos Humanos para la Ciencia y la Tecnología, Fundación Parque Científico y Tecnológico de Castilla-La Mancha, 02006, Albacete, Spain., Osuna A; Department of Parasitology, Biochemical and Molecular Parasitology Group CTS-183, University of Granada, Granada, Spain.; Institute of Biotechnology, University of Granada, Granada, Spain., de Pablos LM; Department of Parasitology, Biochemical and Molecular Parasitology Group CTS-183, University of Granada, Granada, Spain. lpablos@ugr.es.; Institute of Biotechnology, University of Granada, Granada, Spain. lpablos@ugr.es.
Jazyk: angličtina
Zdroj: NPJ biofilms and microbiomes [NPJ Biofilms Microbiomes] 2024 Mar 21; Vol. 10 (1), pp. 27. Date of Electronic Publication: 2024 Mar 21.
DOI: 10.1038/s41522-024-00492-x
Abstrakt: Bees are major pollinators involved in the maintenance of all terrestrial ecosystems. Biotic and abiotic factors placing these insects at risk is a research priority for ecological and agricultural sustainability. Parasites are one of the key players of this global decline and the study of their mechanisms of action is essential to control honeybee colony losses. Trypanosomatid parasites and particularly the Lotmaria passim are widely spread in honeybees, however their lifestyle is poorly understood. In this work, we show how these parasites are able to differentiate into a new parasitic lifestyle: the trypanosomatid biofilms. Using different microscopic techniques, we demonstrated that the secretion of Extracellular Polymeric Substances by free-swimming unicellular promastigote forms is a prerequisite for the generation and adherence of multicellular biofilms to solid surfaces in vitro and in vivo. Moreover, compared to human-infective trypanosomatid parasites our study shows how trypanosomatid parasites of honeybees increases their resistance and thus resilience to drastic changes in environmental conditions such as ultralow temperatures and hypoosmotic shock, which would explain their success thriving within or outside their hosts. These results set up the basis for the understanding of the success of this group of parasites in nature and to unveil the impact of such pathogens in honeybees, a keystones species in most terrestrial ecosystems.
(© 2024. The Author(s).)
Databáze: MEDLINE
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