Acute Administration of HIV-1 Tat Protein Drives Glutamatergic Alterations in a Rodent Model of HIV-Associated Neurocognitive Disorders.

Autor: Duffy BC; Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA, USA.; Center for Molecular Virology and Translational Neuroscience, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, PA, USA., King KM; Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA, USA., Nepal B; Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA, USA., Nonnemacher MR; Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA, USA. mrn25@drexel.edu.; Center for Molecular Virology and Translational Neuroscience, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, PA, USA. mrn25@drexel.edu.; Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, USA. mrn25@drexel.edu., Kortagere S; Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA, USA. sk673@drexel.edu.; Center for Molecular Virology and Translational Neuroscience, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, PA, USA. sk673@drexel.edu.
Jazyk: angličtina
Zdroj: Molecular neurobiology [Mol Neurobiol] 2024 Oct; Vol. 61 (10), pp. 8467-8480. Date of Electronic Publication: 2024 Mar 22.
DOI: 10.1007/s12035-024-04113-8
Abstrakt: HIV-1-associated neurocognitive disorders (HAND) are a major comorbidity of HIV-1 infection, marked by impairment of executive function varying in severity. HAND affects nearly half of people living with HIV (PLWH), with mild forms predominating since the use of anti-retroviral therapies (ART). The HIV-1 transactivator of transcription (Tat) protein is found in the cerebrospinal fluid of patients adherent to ART, and its administration or expression in animals causes cognitive symptoms. Studies of Tat interaction with the N-methyl-D-aspartate receptor (NMDAR) suggest that glutamate toxicity contributes to Tat-induced impairments. To identify changes in regional glutamatergic circuitry underlying cognitive impairment, we injected recombinant Tat86 or saline to medial prefrontal cortex (mPFC) of male Sprague-Dawley rats. Rats were assessed with behavioral tasks that involve intact functioning of mPFC including the novel object recognition (NOR), spatial object recognition (SOR), and temporal order (TO) tasks at 1 and 2 postoperative weeks. Following testing, mPFC tissue was collected and analyzed by RT-PCR. Results showed Tat86 in mPFC-induced impairment in SOR, and upregulation of Grin1 and Grin2a transcripts. To further understand the mechanism of Tat toxicity, we assessed the effects of full-length Tat101 on gene expression in mPFC by RNA sequencing. The results of RNAseq suggest that glutamatergic effects of Tat86 are maintained with Tat101, as Grin2a was upregulated in Tat101-injected tissue, among other differentially expressed genes. Spatial learning and memory impairment and Grin2a upregulation suggest that exposure to Tat protein drives adaptation in mPFC, altering the function of circuitry supporting spatial learning and memory.
(© 2024. The Author(s).)
Databáze: MEDLINE
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