Genetic Diversity Analysis of Anisogramma anomala in the Pacific Northwest and New Jersey.

Autor:	Tobia J; Plant Biology Department, School of Environmental and Biological Science, Rutgers University, New Brunswick, NJ 08901.; Research and Evaluation Group at Public Health Management Corporation, Philadelphia, PA 19102., Muehlbauer M; Rutgers University Cooperative Extension of Hunterdon County, Flemington, NJ 08822-2900., Honig JA; Plant Biology Department, School of Environmental and Biological Science, Rutgers University, New Brunswick, NJ 08901., Pscheidt JW; Department of Botany and Plant Pathology, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331., Hillman BI; Plant Biology Department, School of Environmental and Biological Science, Rutgers University, New Brunswick, NJ 08901., Molnar TJ; Plant Biology Department, School of Environmental and Biological Science, Rutgers University, New Brunswick, NJ 08901.
Jazyk:	angličtina
Zdroj:	Plant disease [Plant Dis] 2024 Aug; Vol. 108 (8), pp. 2422-2434. Date of Electronic Publication: 2024 Jul 25.
DOI:	10.1094/PDIS-08-21-1827-RE
Abstrakt:	Anisogramma anomala , a biotrophic ascomycete, causes eastern filbert blight (EFB) of hazelnuts ( Corylus spp.). EFB is endemic in eastern North America, preventing the commercial production of European hazelnut ( C. avellana L.). In contrast, the historic absence of A. anomala in the Pacific Northwest (PNW) supported the development of a robust hazelnut industry. Circa 1960, A. anomala was inadvertently introduced into southwestern Washington, causing orchard devastation. Distribution of the pathogen in the PNW has been hypothesized to be the result of a single-point introduction. This study aimed to investigate the single-point introduction hypothesis of A. anomala by comparing the genetic diversity of A. anomala samples from the PNW and New Jersey (NJ). Specimens from the main PNW production region ( n = 60) and an area within the pathogen's native range, NJ ( n = 151), were genotyped using 15 simple sequence repeat (SSR) markers. The following were used to assess genetic diversity and population structure: allelic summary statistics, discriminant analysis of principal components, network median-joining tree, analysis of multilocus genotypes, and allelic population diversity analysis. Analyses separated the samples into one cluster containing all the PNW isolates, and five clusters of NJ isolates. The PNW samples were nearly genetically uniform, and the NJ isolates were diverse. These findings support the hypothesis that A. anomala in the PNW was derived from a single-point introduction and corroborate previous studies that have shown A. anomala is very diverse in NJ. This indicates that maintaining restrictions on the movement of Corylus into the PNW is important to prevent the introduction of new populations of A. anomala , thus protecting the PNW hazelnut industry. Competing Interests: The author(s) declare no conflict of interest.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu