Elastic Properties of Aging Human Hematoma Model In Vitro and Its Susceptibility to Histotripsy Liquefaction.

Autor:	Ponomarchuk EM; Physics Faculty, Lomonosov Moscow State University, Moscow, Russia. Electronic address: ponomarchuk.em14@physics.msu.ru., Rosnitskiy PB; Division of Gastroenterology, Department of Medicine, University of Washington School of Medicine, Seattle, WA, USA., Tsysar SA; Physics Faculty, Lomonosov Moscow State University, Moscow, Russia., Khokhlova TD; Division of Gastroenterology, Department of Medicine, University of Washington School of Medicine, Seattle, WA, USA., Karzova MM; Physics Faculty, Lomonosov Moscow State University, Moscow, Russia., Kvashennikova AV; Physics Faculty, Lomonosov Moscow State University, Moscow, Russia., Tumanova KD; Physics Faculty, Lomonosov Moscow State University, Moscow, Russia., Kadrev AV; Department of Urology and Andrology, Medical Research and Educational Center, Lomonosov Moscow State University, Moscow, Russia; Diagnostic Ultrasound Division, Russian Medical Academy of Continuous Professional Education, Moscow, Russia., Buravkov SV; Faculty of Fundamental Medicine, Lomonosov Moscow State University, Moscow, Russia., Trakhtman PE; National Medical Research Center for Pediatric Hematology, Oncology and Immunology, Moscow, Russia., Starostin NN; National Medical Research Center for Pediatric Hematology, Oncology and Immunology, Moscow, Russia., Sapozhnikov OA; Physics Faculty, Lomonosov Moscow State University, Moscow, Russia; Center for Industrial and Medical Ultrasound, Applied Physics Laboratory, University of Washington, Seattle, WA, USA., Khokhlova VA; Physics Faculty, Lomonosov Moscow State University, Moscow, Russia; Center for Industrial and Medical Ultrasound, Applied Physics Laboratory, University of Washington, Seattle, WA, USA.
Jazyk:	angličtina
Zdroj:	Ultrasound in medicine & biology [Ultrasound Med Biol] 2024 Jun; Vol. 50 (6), pp. 927-938. Date of Electronic Publication: 2024 Mar 20.
DOI:	10.1016/j.ultrasmedbio.2024.02.019
Abstrakt:	Objective: Tissue susceptibility to histotripsy disintegration has been reported to depend on its elastic properties. This work was aimed at investigation of histotripsy efficiency for liquefaction of human hematomas, depending on their stiffness and degree of retraction over time (0-10 d). Methods: As an in vitro hematoma model, anticoagulated human blood samples (200 mL) were recalcified at different temperatures. In one set of samples, the shear modulus was measured by shear wave elastography during blood clotting at 10℃, 22℃ and 37℃, and then daily during further aging. The ultrastructure of the samples was analyzed daily with scanning electron microscopy (SEM). Another set of blood samples (50-200 mL) were recalcified at 37℃ for density and retraction measurements over aging and exposed to histotripsy at varying time points. Boiling histotripsy (2.5 ms pulses) and hybrid histotripsy (0.2 ms pulses) exposures (2 MHz, 1% dc, P+/P-/A s = 182/-27/207 MPa in situ) were used to produce either individual cigar-shaped or volumetric (0.8-3 mL) lesions in samples incubated for 3 h, 5 d and 10 d. The obtained lesions were sized, then the lysate aspirated under B-mode guidance was analyzed ultrastructurally and diluted in distilled water for sizing of residual fragments. Results: It was found that clotting time decreased from 113 to 25 min with the increase in blood temperature from 10℃ to 37℃. The shear modulus increased to 0.53 ± 0.17 kPa during clotting and remained constant within 8 d of incubation at 2℃. Sample volumes decreased by 57% because of retraction within 10 d. SEM revealed significant echinocytosis but unchanged ultrastructure of the fibrin meshwork. Liquefaction rate and lesion dimensions produced with the same histotripsy protocols correlated with the increase in the degree of retraction and were lower in retracted samples versus freshly clotted samples. More than 80% of residual fibrin fragments after histotripsy treatment were shorter than 150 µm; the maximum length was 208 µm, allowing for unobstructed aspiration of the lysate with most clinically used needles. Conclusion: The results indicate that hematoma susceptibility to histotripsy liquefaction is not entirely determined by its stiffness, and correlates with the retraction degree. Competing Interests: Conflict of interest The authors declare no competing interests. (Copyright © 2024 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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