A2M possesses anti-bacterial functions by recruiting and enhancing phagocytosis through GRP78 in an echinoderm.
Autor: | Jiang D; State Key Laboratory for Quality and Safety of Agro-products, Ningbo University, Ningbo 315211, PR China., Shao Y; State Key Laboratory for Quality and Safety of Agro-products, Ningbo University, Ningbo 315211, PR China., Zhang S; State Key Laboratory for Quality and Safety of Agro-products, Ningbo University, Ningbo 315211, PR China. Electronic address: zhangsiyuan@nbu.edu.cn., Li C; State Key Laboratory for Quality and Safety of Agro-products, Ningbo University, Ningbo 315211, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, PR China. Electronic address: lichenghua@nbu.edu.cn. |
---|---|
Jazyk: | angličtina |
Zdroj: | International journal of biological macromolecules [Int J Biol Macromol] 2024 Apr; Vol. 265 (Pt 2), pp. 131016. Date of Electronic Publication: 2024 Mar 19. |
DOI: | 10.1016/j.ijbiomac.2024.131016 |
Abstrakt: | Alpha-2-macroglobulin (A2M) is an extracellular macromolecule mainly known for its role as a broad-spectrum protease inhibitor in mammals. However, the immune recognition and regulation mechanisms of A2M in invertebrates are still not well investigated. In the current study, the role of sea cucumber Apostichopus japonicus A2M in the regulation of innate immune responses was explored. We found that AjA2M promotes phagocytosis of Vibrio splendidus in coelomocytes of sea cucumber. Then two major functional structural domains of AjA2M, the thioester domain (TED) and the receptor-binding structural domain (RBD) were cloned. It was found that the AjA2M-TED binds to pathogens while causing Vibrio splendidus aggregation; the AjA2M-RBD interacts with the Glucose Regulated Protein 78 (AjGRP78), subsequently AjGRP78 accelerates the degradation of Vibrio splendidus in lysosomes by facilitating polymerisation and rearrangement of the cytoskeleton. Collectively, the findings together suggest that A2M-GRP78 axis mediates immune signaling pathway of phagocytosis and AjA2M has been characterized to play an essential crucial role in antibacterial immune responses of invertebrates. Competing Interests: Declaration of competing interest The authors declare that they have no conflicts of interest with the contents of this article. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
Databáze: | MEDLINE |
Externí odkaz: |