In Vivo Microrheology Reveals Local Elastic and Plastic Responses Inside 3D Bacterial Biofilms.

Autor: Ohmura T; Biozentrum, University of Basel, Spitalstrasse 41, Basel, 4056, Switzerland., Skinner DJ; Department of Mathematics, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139-4307, USA.; NSF-Simons Center for Quantitative Biology, Northwestern University, Evanston, IL, 60201, USA., Neuhaus K; Biozentrum, University of Basel, Spitalstrasse 41, Basel, 4056, Switzerland.; Department of Physics, Philipps-Universität Marburg, Renthof 5, 35032, Marburg, Germany., Choi GPT; Department of Mathematics, The Chinese University of Hong Kong, N.T., Hong Kong., Dunkel J; Department of Mathematics, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139-4307, USA., Drescher K; Biozentrum, University of Basel, Spitalstrasse 41, Basel, 4056, Switzerland.
Jazyk: angličtina
Zdroj: Advanced materials (Deerfield Beach, Fla.) [Adv Mater] 2024 Jul; Vol. 36 (29), pp. e2314059. Date of Electronic Publication: 2024 May 16.
DOI: 10.1002/adma.202314059
Abstrakt: Bacterial biofilms are highly abundant 3D living materials capable of performing complex biomechanical and biochemical functions, including programmable growth, self-repair, filtration, and bioproduction. Methods to measure internal mechanical properties of biofilms in vivo with spatial resolution on the cellular scale have been lacking. Here, thousands of cells are tracked inside living 3D biofilms of the bacterium Vibrio cholerae during and after the application of shear stress, for a wide range of stress amplitudes, periods, and biofilm sizes, which revealed anisotropic elastic and plastic responses of both cell displacements and cell reorientations. Using cellular tracking to infer parameters of a general mechanical model, spatially-resolved measurements of the elastic modulus inside the biofilm are obtained, which correlate with the spatial distribution of the polysaccharides within the biofilm matrix. The noninvasive microrheology and force-inference approach introduced here provides a general framework for studying mechanical properties with high spatial resolution in living materials.
(© 2024 The Authors. Advanced Materials published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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