Photobiomodulation effects on synovial morphology, iNOS gene, and protein expression in a model of acute inflammation.

Autor:	Bomfim FRCD; Universidade Federal de São Paulo - Escola Paulista de Medicina - Postgraduate Program in Interdisciplinary Surgical Science - São Paulo (SP), Brazil.; Centro Universitário da Fundação Hermínio Ometto - Araras (SP), Brazil., Gomes BS; Universidade Federal de São Paulo - Escola Paulista de Medicina - Postgraduate Program in Interdisciplinary Surgical Science - São Paulo (SP), Brazil., Lanza SZ; Centro Universitário da Fundação Hermínio Ometto - Araras (SP), Brazil., Esquisatto MAM; Centro Universitário da Fundação Hermínio Ometto - Araras (SP), Brazil., Lopes-Filho GJ; Universidade Federal de São Paulo - Escola Paulista de Medicina - Postgraduate Program in Interdisciplinary Surgical Science - São Paulo (SP), Brazil.
Jazyk:	angličtina
Zdroj:	Acta cirurgica brasileira [Acta Cir Bras] 2024 Mar 15; Vol. 39, pp. e392024. Date of Electronic Publication: 2024 Mar 15 (Print Publication: 2024).
DOI:	10.1590/acb392024
Abstrakt:	Purpose: To evaluate morphological aspects and inducible nitric oxide synthase (iNOS) gene and protein expression in a model of acute inflammation. Methods: Thirty-six female Wistar rats were assigned into three groups: control (saline, n = 12), sham (arthritis, n = 12), and PBM (arthritis and photobiomodulation, n = 12). Arthritis induction was performed with 200 μg of intra-articular Zymosan in sham and PBM animals. PBM was performed 24 h after induction with a laser device (λ = 808 nm, 25 mW of nominal power, fluence of 20 J/cm2, beam area of 0.02 mm2, time of 33 s, total energy of 0.825 J) with punctual and single dose application. Morphological analysis of joint structure (HE) and immunohistochemistry (anti-iNOS antibody) were performed on knee samples, and synovial tissue was submitted to RNA extraction, cDNA synthesis and gene expression analysis by quantitative polymerase chain reaction. Statistical analyses were performed with p < 0.05. Results: It was observed an increase in the thickness of the synovial lining epithelium and inflammatory infiltrate in sham compared to PBM. Gene expression analysis showed higher iNOS expression in PBM, and iNOS protein expression decreased in PBM compared to sham. Conclusions: Photobiomodulation decreased inflammation in PBM animals, upregulated iNOS gene expression, however down egulated protein expression compared to sham.
Databáze:	MEDLINE
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