Rok from B. subtilis: Bridging genome structure and transcription regulation.

Autor: Erkelens AM; Leiden Institute of Chemistry, Leiden University, Leiden, the Netherlands.; Centre for Microbial Cell Biology, Leiden University, Leiden, the Netherlands.; Centre for Interdisciplinary Genome Research, Leiden University, Leiden, the Netherlands., van Erp B; Leiden Institute of Chemistry, Leiden University, Leiden, the Netherlands.; Centre for Microbial Cell Biology, Leiden University, Leiden, the Netherlands.; Centre for Interdisciplinary Genome Research, Leiden University, Leiden, the Netherlands., Meijer WJJ; Centro de Biología Molecular Severo Ochoa (CSIC-UAM), C. Nicolás Cabrera 1, Universidad Autónoma, Madrid, Spain., Dame RT; Leiden Institute of Chemistry, Leiden University, Leiden, the Netherlands.; Centre for Microbial Cell Biology, Leiden University, Leiden, the Netherlands.; Centre for Interdisciplinary Genome Research, Leiden University, Leiden, the Netherlands.
Jazyk: angličtina
Zdroj: Molecular microbiology [Mol Microbiol] 2024 Mar 21. Date of Electronic Publication: 2024 Mar 21.
DOI: 10.1111/mmi.15250
Abstrakt: Bacterial genomes are folded and organized into compact yet dynamic structures, called nucleoids. Nucleoid orchestration involves many factors at multiple length scales, such as nucleoid-associated proteins and liquid-liquid phase separation, and has to be compatible with replication and transcription. Possibly, genome organization plays an intrinsic role in transcription regulation, in addition to classical transcription factors. In this review, we provide arguments supporting this view using the Gram-positive bacterium Bacillus subtilis as a model. Proteins BsSMC, HBsu and Rok all impact the structure of the B. subtilis chromosome. Particularly for Rok, there is compelling evidence that it combines its structural function with a role as global gene regulator. Many studies describe either function of Rok, but rarely both are addressed at the same time. Here, we review both sides of the coin and integrate them into one model. Rok forms unusually stable DNA-DNA bridges and this ability likely underlies its repressive effect on transcription by either preventing RNA polymerase from binding to DNA or trapping it inside DNA loops. Partner proteins are needed to change or relieve Rok-mediated gene repression. Lastly, we investigate which features characterize H-NS-like proteins, a family that, at present, lacks a clear definition.
(© 2024 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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