Monocyte-Mediated Thrombosis Linked to Circulating Tissue Factor and Immune Paralysis in COVID-19.

Autor:	Goonewardena SN; Division of Cardiovascular Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor (S.N.G., A.M.T., O.G.G., D.D., P.K.B., S.S.H., D.J.P.)., Chen Q; Metabolism and Lipids Unit, Cardiovascular Institute, Marie-Josee and Henry R. Kravis Center for Cardiovascular Health, Icahn School of Medicine at Mount Sinai, New York, NY (Q.C., R.S.R.)., Tate AM; Division of Cardiovascular Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor (S.N.G., A.M.T., O.G.G., D.D., P.K.B., S.S.H., D.J.P.)., Grushko OG; Division of Cardiovascular Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor (S.N.G., A.M.T., O.G.G., D.D., P.K.B., S.S.H., D.J.P.)., Damodaran D; Division of Cardiovascular Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor (S.N.G., A.M.T., O.G.G., D.D., P.K.B., S.S.H., D.J.P.)., Blakely PK; Division of Cardiovascular Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor (S.N.G., A.M.T., O.G.G., D.D., P.K.B., S.S.H., D.J.P.)., Hayek SS; Division of Cardiovascular Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor (S.N.G., A.M.T., O.G.G., D.D., P.K.B., S.S.H., D.J.P.)., Pinsky DJ; Division of Cardiovascular Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor (S.N.G., A.M.T., O.G.G., D.D., P.K.B., S.S.H., D.J.P.)., Rosenson RS; Metabolism and Lipids Unit, Cardiovascular Institute, Marie-Josee and Henry R. Kravis Center for Cardiovascular Health, Icahn School of Medicine at Mount Sinai, New York, NY (Q.C., R.S.R.).
Jazyk:	angličtina
Zdroj:	Arteriosclerosis, thrombosis, and vascular biology [Arterioscler Thromb Vasc Biol] 2024 May; Vol. 44 (5), pp. 1124-1134. Date of Electronic Publication: 2024 Mar 21.
DOI:	10.1161/ATVBAHA.122.318721
Abstrakt:	Background: SARS-CoV-2 infections cause COVID-19 and are associated with inflammation, coagulopathy, and high incidence of thrombosis. Myeloid cells help coordinate the initial immune response in COVID-19. Although we appreciate that myeloid cells lie at the nexus of inflammation and thrombosis, the mechanisms that unite the two in COVID-19 remain largely unknown. Methods: In this study, we used systems biology approaches including proteomics, transcriptomics, and mass cytometry to define the circulating proteome and circulating immune cell phenotypes in subjects with COVID-19. Results: In a cohort of subjects with COVID-19 (n=35), circulating markers of inflammation (CCL23 [C-C motif chemokine ligand 23] and IL [interleukin]-6) and vascular dysfunction (ACE2 [angiotensin-converting enzyme 2] and TF [tissue factor]) were elevated in subjects with severe compared with mild COVID-19. Additionally, although the total white blood cell counts were similar between COVID-19 groups, CD14+ (cluster of differentiation) monocytes from subjects with severe COVID-19 expressed more TF. At baseline, transcriptomics demonstrated increased IL-6, CCL3, ACOD1 (aconitate decarboxylase 1), C5AR1 (complement component 5a receptor), C5AR2, and TF in subjects with severe COVID-19 compared with controls. Using stress transcriptomics, we found that circulating immune cells from subjects with severe COVID-19 had evidence of profound immune paralysis with greatly reduced transcriptional activation and release of inflammatory markers in response to TLR (Toll-like receptor) activation. Finally, sera from subjects with severe (but not mild) COVID-19 activated human monocytes and induced TF expression. Conclusions: Taken together, these observations further elucidate the pathological mechanisms that underlie immune dysfunction and coagulation abnormalities in COVID-19, contributing to our growing understanding of SARS-CoV-2 infections that could also be leveraged to develop novel diagnostic and therapeutic strategies. Competing Interests: Disclosures None.
Databáze:	MEDLINE
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