Annotation-free learning of a spatio-temporal manifold of the cell life cycle.

Autor:	Delas Peñas K; Department of Engineering Science, University of Oxford, Oxford, United Kingdom.; Big Data Institute, Li Ka Shing Centre for Health Information and Discovery, University of Oxford, Oxford, United Kingdom.; Department of Computer Science, University of the Philippines, Quezon City, Philippines., Dmitrieva M; Department of Engineering Science, University of Oxford, Oxford, United Kingdom.; Big Data Institute, Li Ka Shing Centre for Health Information and Discovery, University of Oxford, Oxford, United Kingdom., Waithe D; WIMM Centre for Computational Biology, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom., Rittscher J; Department of Engineering Science, University of Oxford, Oxford, United Kingdom.; Big Data Institute, Li Ka Shing Centre for Health Information and Discovery, University of Oxford, Oxford, United Kingdom.; Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
Jazyk:	angličtina
Zdroj:	Biological imaging [Biol Imaging] 2023 Oct 06; Vol. 3, pp. e19. Date of Electronic Publication: 2023 Oct 06 (Print Publication: 2023).
DOI:	10.1017/S2633903X23000193
Abstrakt:	The cell cycle is a complex biological phenomenon, which plays an important role in many cell biological processes and disease states. Machine learning is emerging to be a pivotal technique for the study of the cell cycle, resulting in a number of available tools and models for the analysis of the cell cycle. Most, however, heavily rely on expert annotations, prior knowledge of mechanisms, and imaging with several fluorescent markers to train their models. Many are also limited to processing only the spatial information in the cell images. In this work, we describe a different approach based on representation learning to construct a manifold of the cell life cycle. We trained our model such that the representations are learned without exhaustive annotations nor assumptions. Moreover, our model uses microscopy images derived from a single fluorescence channel and utilizes both the spatial and temporal information in these images. We show that even with fewer channels and self-supervision, information relevant to cell cycle analysis such as staging and estimation of cycle duration can still be extracted, which demonstrates the potential of our approach to aid future cell cycle studies and in discovery cell biology to probe and understand novel dynamic systems. Competing Interests: The authors declare none. (© The Author(s) 2023.)
Databáze:	MEDLINE
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