The genome of the colonial hydroid Hydractinia reveals that their stem cells use a toolkit of evolutionarily shared genes with all animals.

Autor:	Schnitzler CE; Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, Florida 32080, USA.; Department of Biology, University of Florida, Gainesville, Florida 32611, USA., Chang ES; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20892, USA., Waletich J; Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, Florida 32080, USA.; Department of Biology, University of Florida, Gainesville, Florida 32611, USA., Quiroga-Artigas G; Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, Florida 32080, USA.; Department of Biology, University of Florida, Gainesville, Florida 32611, USA.; Centre de Recherche en Biologie cellulaire de Montpellier (CRBM), Université de Montpellier, Centre National de la Recherche Scientifique, 34293 Montpellier CEDEX 05, France., Wong WY; Department for Neurosciences and Developmental Biology, University of Vienna, 1030 Vienna, Austria., Nguyen AD; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA., Barreira SN; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA., Doonan LB; Centre for Chromosome Biology, College of Science and Engineering, University of Galway, Galway H91 W2TY, Ireland., Gonzalez P; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA., Koren S; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA., Gahan JM; Centre for Chromosome Biology, College of Science and Engineering, University of Galway, Galway H91 W2TY, Ireland.; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom., Sanders SM; Department of Surgery, Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA.; Pittsburgh Center for Evolutionary Biology and Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA., Bradshaw B; Centre for Chromosome Biology, College of Science and Engineering, University of Galway, Galway H91 W2TY, Ireland., DuBuc TQ; Centre for Chromosome Biology, College of Science and Engineering, University of Galway, Galway H91 W2TY, Ireland.; Department of Biology, Swarthmore College, Swarthmore, Pennsylvania 19081, USA., Febrimarsa; Centre for Chromosome Biology, College of Science and Engineering, University of Galway, Galway H91 W2TY, Ireland.; Pharmaceutical Biology Laboratory, Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Jawa Tengah 57169, Indonesia., de Jong D; Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, Florida 32080, USA.; Department of Biology, University of Florida, Gainesville, Florida 32611, USA., Nawrocki EP; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20892, USA., Larson A; Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, Florida 32080, USA., Klasfeld S; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA., Gornik SG; Centre for Chromosome Biology, College of Science and Engineering, University of Galway, Galway H91 W2TY, Ireland.; Center for Organismal Studies, University of Heidelberg, 69117 Heidelberg, Germany., Moreland RT; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA., Wolfsberg TG; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA., Phillippy AM; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA., Mullikin JC; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.; NIH Intramural Sequencing Center, Rockville, Maryland 20852, USA., Simakov O; Department for Neurosciences and Developmental Biology, University of Vienna, 1030 Vienna, Austria., Cartwright P; Department of Evolution and Ecology, University of Kansas, Lawrence, Kansas 66045, USA., Nicotra M; Department of Surgery, Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA.; Pittsburgh Center for Evolutionary Biology and Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA., Frank U; Centre for Chromosome Biology, College of Science and Engineering, University of Galway, Galway H91 W2TY, Ireland., Baxevanis AD; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA; andy@mail.nih.gov.
Jazyk:	angličtina
Zdroj:	Genome research [Genome Res] 2024 Apr 25; Vol. 34 (3), pp. 498-513. Date of Electronic Publication: 2024 Apr 25.
DOI:	10.1101/gr.278382.123
Abstrakt:	Hydractinia is a colonial marine hydroid that shows remarkable biological properties, including the capacity to regenerate its entire body throughout its lifetime, a process made possible by its adult migratory stem cells, known as i-cells. Here, we provide an in-depth characterization of the genomic structure and gene content of two Hydractinia species, Hydractinia symbiolongicarpus and Hydractinia echinata , placing them in a comparative evolutionary framework with other cnidarian genomes. We also generated and annotated a single-cell transcriptomic atlas for adult male H. symbiolongicarpus and identified cell-type markers for all major cell types, including key i-cell markers. Orthology analyses based on the markers revealed that Hydractinia 's i-cells are highly enriched in genes that are widely shared amongst animals, a striking finding given that Hydractinia has a higher proportion of phylum-specific genes than any of the other 41 animals in our orthology analysis. These results indicate that Hydractinia 's stem cells and early progenitor cells may use a toolkit shared with all animals, making it a promising model organism for future exploration of stem cell biology and regenerative medicine. The genomic and transcriptomic resources for Hydractinia presented here will enable further studies of their regenerative capacity, colonial morphology, and ability to distinguish self from nonself. (© 2024 Schnitzler et al.; Published by Cold Spring Harbor Laboratory Press.)
Databáze:	MEDLINE
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