BacA: a possible regulator that contributes to the biofilm formation of Pseudomonas aeruginosa .

Autor:	Wallart L; Univ Rouen Normandie, INSA Rouen Normandie, CNRS, Normandie Univ, PBS UMR 6270, Rouen, France., Ben Mlouka MA; Univ Rouen Normandie, INSA Rouen Normandie, CNRS, Normandie Univ, PBS UMR 6270, Rouen, France.; Univ Rouen Normandy, INSERM US 51, CNRS UAR 2026, HeRacLeS PISSARO, Rouen, France., Saffiedine B; Univ Rouen Normandie, INSA Rouen Normandie, CNRS, Normandie Univ, PBS UMR 6270, Rouen, France., Coquet L; Univ Rouen Normandie, INSA Rouen Normandie, CNRS, Normandie Univ, PBS UMR 6270, Rouen, France.; Univ Rouen Normandy, INSERM US 51, CNRS UAR 2026, HeRacLeS PISSARO, Rouen, France., Le H; Univ Rouen Normandie, INSA Rouen Normandie, CNRS, Normandie Univ, PBS UMR 6270, Rouen, France.; Univ Rouen Normandy, INSERM US 51, CNRS UAR 2026, HeRacLeS PISSARO, Rouen, France., Hardouin J; Univ Rouen Normandie, INSA Rouen Normandie, CNRS, Normandie Univ, PBS UMR 6270, Rouen, France.; Univ Rouen Normandy, INSERM US 51, CNRS UAR 2026, HeRacLeS PISSARO, Rouen, France., Jouenne T; Univ Rouen Normandie, INSA Rouen Normandie, CNRS, Normandie Univ, PBS UMR 6270, Rouen, France., Phan G; Paris Cité University, CiTCoM, CNRS, Paris, France., Kiefer-Meyer MC; Univ Rouen Normandie, Normandie Univ, GlycoMEV UR 4358, SFR Normandie Végétal FED 4277, Innovation Chimie Carnot, RMT BESTIM, GDR Chemobiologie, IRIB, Rouen, France., Girard E; Grenoble Alpes University, CNRS, CEA, IBS, Grenoble, France., Broutin I; Paris Cité University, CiTCoM, CNRS, Paris, France., Cosette P; Univ Rouen Normandie, INSA Rouen Normandie, CNRS, Normandie Univ, PBS UMR 6270, Rouen, France.; Univ Rouen Normandy, INSERM US 51, CNRS UAR 2026, HeRacLeS PISSARO, Rouen, France.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2024 Mar 05; Vol. 15, pp. 1332448. Date of Electronic Publication: 2024 Mar 05 (Print Publication: 2024).
DOI:	10.3389/fmicb.2024.1332448
Abstrakt:	Previously, we pointed out in P. aeruginosa PAO1 biofilm cells the accumulation of a hypothetical protein named PA3731 and showed that the deletion of the corresponding gene impacted its biofilm formation capacity. PA3731 belongs to a cluster of 4 genes ( pa3732 to pa3729 ) that we named bac for "Biofilm Associated Cluster." The present study focuses on the PA14_16140 protein, i.e., the PA3732 (BacA) homolog in the PA14 strain. The role of BacA in rhamnolipid secretion, biofilm formation and virulence, was confirmed by phenotypic experiments with a bacA mutant. Additional investigations allow to advance that the bac system involves in fact 6 genes organized in operon, i.e., bacA to bacF. At a molecular level, quantitative proteomic studies revealed an accumulation of the BAC cognate partners by the bacA sessile mutant, suggesting a negative control of BacA toward the bac operon. Finally, a first crystallographic structure of BacA was obtained revealing a structure homologous to chaperones or/and regulatory proteins. Competing Interests: EG declares a potential conflict of interest since he is co-founder of the Polyvalan company that commercializes the crystallophore. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Wallart, Ben Mlouka, Saffiedine, Coquet, Le, Hardouin, Jouenne, Phan, Kiefer-Meyer, Girard, Broutin and Cosette.)
Databáze:	MEDLINE
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