Transmembrane Shuttling of Photosynthetically Produced Electrons to Propel Extracellular Biocatalytic Redox Reactions in a Modular Fashion.

Autor: Jurkaš V; Institute of Chemistry University of Graz Heinrichstraße 28 8010 Graz Austria., Weissensteiner F; Institute of Chemistry University of Graz Heinrichstraße 28 8010 Graz Austria., De Santis P; Institute of Chemistry University of Graz Heinrichstraße 28 8010 Graz Austria.; Department of Engineering, Biological and Chemical Engineering Section Biocatalysis and Bioprocessing Group Aarhus University Gustav Wieds Vej 10 8000 Aarhus Denmark., Vrabl S; Institute of Chemistry University of Graz Heinrichstraße 28 8010 Graz Austria., Sorgenfrei FA; Austrian Centre of Industrial Biotechnology, c/o Institute of Chemistry, University of Graz Heinrichstraße 28 8010 Graz Austria., Bierbaumer S; Institute of Chemistry University of Graz Heinrichstraße 28 8010 Graz Austria., Kara S; Department of Engineering, Biological and Chemical Engineering Section Biocatalysis and Bioprocessing Group Aarhus University Gustav Wieds Vej 10 8000 Aarhus Denmark., Kourist R; Institute of Molecular Biotechnology Graz University of Technology Petersgasse 14 8010 Graz Austria., Wangikar PP; Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076 India DBT-Pan IIT Centre for Bioenergy, Indian Institute of Technology Bombay, Powai, Mumbai 400076 India Wadhwani Research Centre for Bioengineering Indian Institute of Technology Bombay Powai Mumbai 400076 India., Winkler CK; Institute of Chemistry University of Graz Heinrichstraße 28 8010 Graz Austria., Kroutil W; Institute of Chemistry University of Graz Heinrichstraße 28 8010 Graz Austria.; Field of Excellence BioHealth- University of Graz 8010 Graz Austria.; BioTechMed Graz 8010 Graz Austria.
Jazyk: angličtina
Zdroj: Angewandte Chemie (Weinheim an der Bergstrasse, Germany) [Angew Chem Weinheim Bergstr Ger] 2022 Oct 04; Vol. 134 (40), pp. e202207971. Date of Electronic Publication: 2022 Aug 29.
DOI: 10.1002/ange.202207971
Abstrakt: Many biocatalytic redox reactions depend on the cofactor NAD(P)H, which may be provided by dedicated recycling systems. Exploiting light and water for NADPH-regeneration as it is performed, e.g. by cyanobacteria, is conceptually very appealing due to its high atom economy. However, the current use of cyanobacteria is limited, e.g. by challenging and time-consuming heterologous enzyme expression in cyanobacteria as well as limitations of substrate or product transport through the cell wall. Here we establish a transmembrane electron shuttling system propelled by the cyanobacterial photosynthesis to drive extracellular NAD(P)H-dependent redox reactions. The modular photo-electron shuttling (MPS) overcomes the need for cloning and problems associated with enzyme- or substrate-toxicity and substrate uptake. The MPS was demonstrated on four classes of enzymes with 19 enzymes and various types of substrates, reaching conversions of up to 99 % and giving products with >99 % optical purity.
Competing Interests: Valentina Jurkaš, Florian Weissensteiner, Sarah Bierbaumer, Christoph K. Winkler and Wolfgang Kroutil are authors of a patent application: Austrian priority application, filed 18/03/2021. A55/2021
(© 2022 The Authors. Angewandte Chemie published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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