BRAF p.V600E Mutational Status Does Not Correlate with Biological Behavior in Conventional Ameloblastomas: A Disease-Free Survival Analysis.

Autor: Martins-de-Barros AV; Post-Graduation Program in Dentistry, School of Dentistry, University of Pernambuco (UPE), Recife, Pernambuco, Brazil., da Costa Araújo FA; Post-Graduation Program in Dentistry, School of Dentistry, University of Pernambuco (UPE), Recife, Pernambuco, Brazil. fabio.andrey@upe.br.; Department of Oral and Maxillofacial Surgery, Hospital Universitário Oswaldo Cruz (HUOC/UPE), Recife, Pernambuco, Brazil. fabio.andrey@upe.br.; Department of Oral and Maxillofacial Surgery, School of Dentistry, University of Pernambuco, Rua Arnóbio Marquês, 310, Santo Amaro, Recife, Pernambuco, 50100-130, Brazil. fabio.andrey@upe.br., Faro TF; Post-Graduation Program in Dentistry, School of Dentistry, University of Pernambuco (UPE), Recife, Pernambuco, Brazil., de Aquino AAT; Undergraduate Program in Dentistry, School of Dentistry, University of Pernambuco (UPE), Recife, Pernambuco, Brazil., Barbosa Neto AG; Instituto de Ciências Biológicas (ICB/UPE), University of Pernambuco (UPE), Recife, Pernambuco, Brazil., da Silva HAM; Instituto de Ciências Biológicas (ICB/UPE), University of Pernambuco (UPE), Recife, Pernambuco, Brazil., de Lima ELS; Instituto de Ciências Biológicas (ICB/UPE), University of Pernambuco (UPE), Recife, Pernambuco, Brazil., Muniz MTC; Instituto de Ciências Biológicas (ICB/UPE), University of Pernambuco (UPE), Recife, Pernambuco, Brazil., de Oliveira E Silva ED; Post-Graduation Program in Dentistry, School of Dentistry, University of Pernambuco (UPE), Recife, Pernambuco, Brazil.; Department of Oral and Maxillofacial Surgery, Hospital Universitário Oswaldo Cruz (HUOC/UPE), Recife, Pernambuco, Brazil., de Vasconcelos Carvalho M; Post-Graduation Program in Dentistry, School of Dentistry, University of Pernambuco (UPE), Recife, Pernambuco, Brazil.; Centro Integrado de Anatomia Patológica (CIAP), Hospital Universitário Oswaldo Cruz (HUOC/UPE), Recife, Pernambuco, Brazil.
Jazyk: angličtina
Zdroj: Head and neck pathology [Head Neck Pathol] 2024 Mar 19; Vol. 18 (1), pp. 23. Date of Electronic Publication: 2024 Mar 19.
DOI: 10.1007/s12105-024-01621-w
Abstrakt: Background: Dysregulation of the MAPK pathway appears to exert a pivotal role in the pathogenesis of ameloblastomas, since BRAF p.V600E has been reported in over 65% of the tumors. Therefore, the purpose of this study was to investigate whether the BRAF p.V600E is related to biological behavior and disease-free survival in patients with conventional ameloblastomas.
Methods: This is a retrospective cohort study based on the STROBE (Strengthening the Reporting of Observational Studies in Epidemiology) recommendations. The study population consisted of individuals treated for conventional ameloblastomas. Clinical, imaging, histomorphological, immunohistochemical (Ki67 and CD138/syndecan-1), and molecular BRAF p.V600E mutation analyses were performed. Bivariate statistical analysis was performed through chi-square and Fisher's exact tests. Kaplan-Meier analysis with log-rank test and Cox proportional hazards regression were used to identify predictors of disease-free survival, with a significance level of 5%.
Results: Forty-one individuals were included, with a male-to-female ratio of 1.15:1. BRAF p.V600E mutation was identified in 75.6% of the tumors. No association between the BRAF mutational status and other clinical, imaging, histomorphological, and immunohistochemical variables was observed. Only the initial treatment modality was significantly associated with a better prognosis in univariate (p = 0.008) and multivariate (p = 0.030) analyses, with a hazard ratio of 9.60 (95%IC = 1.24-73.89), favoring radical treatment.
Conclusion: BRAF p.V600E mutation emerges as a prevalent molecular aberration in ameloblastomas. Nevertheless, it does not seem to significantly affect the tumor proliferative activity, CD138/syndecan-1-mediated cell adhesion, or disease-free survival outcomes.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: