Performance evaluation of Trichoderma reseei in tolerance and biodegradation of diuron herbicide in agar plate, liquid culture and solid-state fermentation.

Autor: Reyes-Cervantes A; Posgrado en Biotecnología, Ex-Hacienda de Santa Bárbara, Universidad Politécnica de Pachuca, Mpio., Carretera Pachuca Cd. Sahagún Km. 20, C.P. 43830, Zempoala, Hgo, Mexico., Robles-Morales DL; Posgrado en Biotecnología, Ex-Hacienda de Santa Bárbara, Universidad Politécnica de Pachuca, Mpio., Carretera Pachuca Cd. Sahagún Km. 20, C.P. 43830, Zempoala, Hgo, Mexico., Tec-Caamal EN; Centre of Bioengineering, School of Engineering and Sciences, Tecnologico de Monterrey, Campus Querétaro, Av. Epigmenio González 500, 76130, Santiago de Querétaro, Querétaro, Mexico., Jiménez-González A; Posgrado en Biotecnología, Ex-Hacienda de Santa Bárbara, Universidad Politécnica de Pachuca, Mpio., Carretera Pachuca Cd. Sahagún Km. 20, C.P. 43830, Zempoala, Hgo, Mexico., Medina-Moreno SA; Posgrado en Biotecnología, Ex-Hacienda de Santa Bárbara, Universidad Politécnica de Pachuca, Mpio., Carretera Pachuca Cd. Sahagún Km. 20, C.P. 43830, Zempoala, Hgo, Mexico. samm67@upp.edu.mx.; Centre of Bioengineering, School of Engineering and Sciences, Tecnologico de Monterrey, Campus Querétaro, Av. Epigmenio González 500, 76130, Santiago de Querétaro, Querétaro, Mexico. samm67@upp.edu.mx.
Jazyk: angličtina
Zdroj: World journal of microbiology & biotechnology [World J Microbiol Biotechnol] 2024 Mar 20; Vol. 40 (5), pp. 137. Date of Electronic Publication: 2024 Mar 20.
DOI: 10.1007/s11274-024-03931-1
Abstrakt: The present study evaluated the performance of the fungus Trichoderma reesei to tolerate and biodegrade the herbicide diuron in its agrochemical presentation in agar plates, liquid culture, and solid-state fermentation. The tolerance of T. reesei to diuron was characterized through a non-competitive inhibition model of the fungal radial growth on the PDA agar plate and growth in liquid culture with glucose and ammonium nitrate, showing a higher tolerance to diuron on the PDA agar plate (inhibition constant 98.63 mg L -1 ) than in liquid culture (inhibition constant 39.4 mg L -1 ). Diuron biodegradation by T. reesei was characterized through model inhibition by the substrate on agar plate and liquid culture. In liquid culture, the fungus biotransformed diuron into 3,4-dichloroaniline using the amide group from the diuron structure as a carbon and nitrogen source, yielding 0.154 mg of biomass per mg of diuron. A mixture of barley straw and agrolite was used as the support and substrate for solid-state fermentation. The diuron removal percentage in solid-state fermentation was fitted by non-multiple linear regression to a parabolic surface response model and reached the higher removal (97.26%) with a specific aeration rate of 1.0 vkgm and inoculum of 2.6 × 10 8 spores g -1 . The diuron removal in solid-state fermentation by sorption on barley straw and agrolite was discarded compared to the removal magnitude of the biosorption and biodegradation mechanisms of Trichoderma reesei. The findings in this work about the tolerance and capability of Trichoderma reesei to remove diuron in liquid and solid culture media demonstrate the potential of the fungus to be implemented in bioremediation technologies of herbicide-polluted sites.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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