Antibiotics and probiotics-induced effects on the total fatty acid composition of feces in a rat model.

Autor: Marosvölgyi T; Institute of Bioanalysis, Medical School, University of Pécs, Pécs, 7624, Hungary., Mintál K; Institute of Physiology, Medical School, University of Pécs, Pécs, 7624, Hungary.; Medical and Engineering Multidisciplinary Cellular Bioimpedance Research Group, Szentágothai Research Centre, University of Pécs, Pécs, 7624, Hungary., Farkas N; Institute of Bioanalysis, Medical School, University of Pécs, Pécs, 7624, Hungary., Sipos Z; Institute of Bioanalysis, Medical School, University of Pécs, Pécs, 7624, Hungary., Makszin L; Institute of Bioanalysis, Medical School, University of Pécs, Pécs, 7624, Hungary., Szabó É; Department of Biochemistry and Medical Chemistry, Medical School, University of Pécs, Pécs, 7624, Hungary. szabo.eva.dr@pte.hu., Tóth A; Institute of Physiology, Medical School, University of Pécs, Pécs, 7624, Hungary.; Medical and Engineering Multidisciplinary Cellular Bioimpedance Research Group, Szentágothai Research Centre, University of Pécs, Pécs, 7624, Hungary., Kocsis B; Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, 7624, Hungary., Kovács K; Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, 7624, Hungary., Hormay E; Institute of Physiology, Medical School, University of Pécs, Pécs, 7624, Hungary.; Medical and Engineering Multidisciplinary Cellular Bioimpedance Research Group, Szentágothai Research Centre, University of Pécs, Pécs, 7624, Hungary., Lénárd L; Institute of Physiology, Medical School, University of Pécs, Pécs, 7624, Hungary.; Medical and Engineering Multidisciplinary Cellular Bioimpedance Research Group, Szentágothai Research Centre, University of Pécs, Pécs, 7624, Hungary., Karádi Z; Institute of Physiology, Medical School, University of Pécs, Pécs, 7624, Hungary.; Medical and Engineering Multidisciplinary Cellular Bioimpedance Research Group, Szentágothai Research Centre, University of Pécs, Pécs, 7624, Hungary., Bufa A; Institute of Bioanalysis, Medical School, University of Pécs, Pécs, 7624, Hungary.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Mar 19; Vol. 14 (1), pp. 6542. Date of Electronic Publication: 2024 Mar 19.
DOI: 10.1038/s41598-024-57046-6
Abstrakt: Fatty acids (FAs) play important roles as membrane components and signal transduction molecules. Changes in short chain FA (SCFA) composition are associated with gut microbiota modifications. However, the effect of bacteria-driven changes on the detailed FA spectrum has not been explored yet. We investigated the effect of antibiotics (ABx) and/or probiotics, in four treatment groups on rat stool FA composition. Principal component analysis indicated that the chromatogram profiles of the treatment groups differ, which was also observed at different time points. Linear mixed effects models showed that in the parameters compared (sampling times, treatments. and their interactions), both the weight percentage and the concentration of FAs were affected by ABx and probiotic administration. This study found that the gut microbiome defines trans and branched saturated FAs, most saturated FAs, and unsaturated FAs with less carbon atoms. These results are among the first ones to demonstrate the restoring effects of a probiotic mixture on a substantial part of the altered total FA spectrum, and also revealed a previously unknown relationship between gut bacteria and a larger group of FAs. These findings suggest that intestinal bacteria produce not only SCFAs but also other FAs that may affect the host's physiological processes.
(© 2024. The Author(s).)
Databáze: MEDLINE
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