From antioxidant defense to genotoxicity: Deciphering the tissue-specific impact of AgNPs on marine clam Ruditapes philippinarum.

Autor: Xing YY; Key Laboratory of Environment and Ecology (Ministry of Education), Ocean University of China, Qingdao, Shandong 266100, PR China; Research Center of Marine Ecology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, Shandong 266061, PR China., Pu XM; Research Center of Marine Ecology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, Shandong 266061, PR China; Laboratory for Marine Ecology and Environmental Science, Laoshan Laboratory, Qingdao, Shandong 266200, PR China. Electronic address: xmpu@fio.org.cn., Pan JF; Key Laboratory of Environment and Ecology (Ministry of Education), Ocean University of China, Qingdao, Shandong 266100, PR China; Laboratory for Marine Ecology and Environmental Science, Laoshan Laboratory, Qingdao, Shandong 266200, PR China; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering, Ocean University of China, Qingdao 266100, PR China. Electronic address: jfpan@ouc.edu.cn., Xu JY; Key Laboratory of Environment and Ecology (Ministry of Education), Ocean University of China, Qingdao, Shandong 266100, PR China; Research Center of Marine Ecology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, Shandong 266061, PR China., Liu C; Key Laboratory of Environment and Ecology (Ministry of Education), Ocean University of China, Qingdao, Shandong 266100, PR China; Research Center of Marine Ecology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, Shandong 266061, PR China; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering, Ocean University of China, Qingdao 266100, PR China., Lu DC; Key Laboratory of Environment and Ecology (Ministry of Education), Ocean University of China, Qingdao, Shandong 266100, PR China; Research Center of Marine Ecology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, Shandong 266061, PR China; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering, Ocean University of China, Qingdao 266100, PR China.
Jazyk: angličtina
Zdroj: Aquatic toxicology (Amsterdam, Netherlands) [Aquat Toxicol] 2024 May; Vol. 270, pp. 106883. Date of Electronic Publication: 2024 Mar 12.
DOI: 10.1016/j.aquatox.2024.106883
Abstrakt: The escalating use of silver nanoparticles (AgNPs) across various sectors for their broad-spectrum antimicrobial capabilities, has raised concern over their potential ecotoxicological effects on aquatic life. This study explores the impact of AgNPs (50 μg/L) on the marine clam Ruditapes philippinarum, with a particular focus on its gills and digestive glands. We adopted an integrated approach that combined in vivo exposure, biochemical assays, and transcriptomic analysis to evaluate the toxicity of AgNPs. The results revealed substantial accumulation of AgNPs in the gills and digestive glands of R. philippinarum, resulting in oxidative stress and DNA damage, with the gills showing more severe oxidative damage. Transcriptomic analysis further highlights an adaptive up-regulation of peroxisome-related genes in the gills responding to AgNP-induxed oxidative stress. Additionally, there was a noteworthy enrichment of differentially expressed genes (DEGs) in key biological processes, including ion binding, NF-kappa B signaling and cytochrome P450-mediated metabolism of xenobiotics. These insights elucidate the toxicological mechanisms of AgNPs to R. philippinarum, emphasizing the gill as a potential sensitive organ for monitoring emerging nanopollutants. Overall, this study significantly advances our understanding of the mechanisms driving nanoparticle-induced stress responses in bivalves and lays the groundwork for future investigations into preventing and treating such pollutants in aquaculture.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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