Fluorescent In Situ Detection of Small RNAs in Plants Using sRNA-FISH.

Autor:	Huang K; Department of Plant and Soil Sciences, University of Delaware, Newark, DE, USA.; Bio-Imaging Center, Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA.; Molecular Imaging Core, Dana-Farber Cancer Institute, Boston, USA., Meyers BC; Department of Plant and Soil Sciences, University of Delaware, Newark, DE, USA. bmeyers@danforthcenter.org.; Donald Danforth Plant Science Center, St. Louis, MO, USA. bmeyers@danforthcenter.org.; Division of Plant Science and Technology, University of Missouri - Columbia, Columbia, MO, USA. bmeyers@danforthcenter.org., Caplan JL; Department of Plant and Soil Sciences, University of Delaware, Newark, DE, USA. jcaplan@udel.edu.; Bio-Imaging Center, Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA. jcaplan@udel.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2784, pp. 101-111.
DOI:	10.1007/978-1-0716-3766-1_7
Abstrakt:	Plant small RNAs are 21-24 nucleotide, noncoding RNAs that function as regulators in plant growth and development. Colorimetric detection of plant small RNAs was made possible with the introduction of locked nucleic acid probes. However, fluorescent detection of plant small RNAs has been challenging due to the high autofluorescence from plant tissue. Here we report a fluorescent in situ detection method for plant small RNAs. This method can be applied to most plant samples and tissue types and also can be adapted for single-molecule detection of small RNAs with super-resolution microscopy. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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