| Autor: |
Zou Q; Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China. liukechun2000@163.com., Duan H; School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China., Fang S; School of Pharmaceutical sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China., Sheng W; Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China. liukechun2000@163.com., Li X; Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China. liukechun2000@163.com., Stoika R; Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine., Finiuk N; Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine., Panchuk R; Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine., Liu K; Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China. liukechun2000@163.com., Wang L; Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China. liukechun2000@163.com. |
| Abstrakt: |
Particles with a porous structure can lead to quick hemostasis and provide a good matrix for cell proliferation during wound healing. Recently, many particle-based wound healing materials have been clinically applied. However, these products show good hemostatic ability but with poor wound healing ability. To solve this problem, this study fabricated APGG composite particles using yeast β-glucan (obtained from Saccharomyces cerevisiae ), sodium alginate, and γ-polyglutamic acid as the starting materials. The structure of yeast β-glucan was modified with many carboxymethyl groups to obtain carboxymethylated β-glucan, which could coordinate with Ca 2+ ions to form a crosslinked structure. A morphology study indicated that the APGG particles showed an irregular spheroidal structure with a low density (<0.1 g cm -3 ) and high porosity (>40%). An in vitro study revealed that the particles exhibited a low BCI value, low hemolysis ratio, and good cytocompatibility against L929 cells. The APGG particles could quickly stop bleeding in a mouse liver injury model and exhibited better hemostatic ability than the commercially available product Celox. Furthermore, the APGG particles could accelerate the healing of non-infected wounds, and the expression levels of CD31, α-SMA, and VEGF related to angiogenesis were significantly enhanced. |
