Engineering Escherichia coli for high-yielding 2,5-Dimethylpyrazine synthesis from L-Threonine by reconstructing metabolic pathways and enhancing cofactors regeneration.

Autor: Liu XX; Henan Provincial Engineering Laboratory of Insect Bio-Reactor, Henan International Joint Laboratory of Insect Biology and Henan Key Laboratory of Insect Biology in Funiu Mountain, Nanyang Normal University, 1638 Wolong Road, Nanyang, Henan, 473061, People's Republic of China., Wang Y; Henan Provincial Engineering Laboratory of Insect Bio-Reactor, Henan International Joint Laboratory of Insect Biology and Henan Key Laboratory of Insect Biology in Funiu Mountain, Nanyang Normal University, 1638 Wolong Road, Nanyang, Henan, 473061, People's Republic of China., Zhang JH; Postdoctoral Innovation Practice Base, She Dian Lao Jiu Co. Ltd., 2 Liquor Avenue, Nanyang, Henan, 473300, People's Republic of China., Lu YF; Henan Provincial Engineering Laboratory of Insect Bio-Reactor, Henan International Joint Laboratory of Insect Biology and Henan Key Laboratory of Insect Biology in Funiu Mountain, Nanyang Normal University, 1638 Wolong Road, Nanyang, Henan, 473061, People's Republic of China., Dong ZX; Henan Provincial Engineering Laboratory of Insect Bio-Reactor, Henan International Joint Laboratory of Insect Biology and Henan Key Laboratory of Insect Biology in Funiu Mountain, Nanyang Normal University, 1638 Wolong Road, Nanyang, Henan, 473061, People's Republic of China., Yue C; Henan Provincial Engineering Laboratory of Insect Bio-Reactor, Henan International Joint Laboratory of Insect Biology and Henan Key Laboratory of Insect Biology in Funiu Mountain, Nanyang Normal University, 1638 Wolong Road, Nanyang, Henan, 473061, People's Republic of China., Huang XQ; College of Food Science and Technology, Henan Agricultural University, 63 Agricultural Road, Zhengzhou, Henan, 450002, People's Republic of China., Zhang SP; Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China., Li DD; Henan Provincial Engineering Laboratory of Insect Bio-Reactor, Henan International Joint Laboratory of Insect Biology and Henan Key Laboratory of Insect Biology in Funiu Mountain, Nanyang Normal University, 1638 Wolong Road, Nanyang, Henan, 473061, People's Republic of China. lidannytc@126.com., Yao LG; Henan Provincial Engineering Laboratory of Insect Bio-Reactor, Henan International Joint Laboratory of Insect Biology and Henan Key Laboratory of Insect Biology in Funiu Mountain, Nanyang Normal University, 1638 Wolong Road, Nanyang, Henan, 473061, People's Republic of China. lunguangyao@163.com., Tang CD; Henan Provincial Engineering Laboratory of Insect Bio-Reactor, Henan International Joint Laboratory of Insect Biology and Henan Key Laboratory of Insect Biology in Funiu Mountain, Nanyang Normal University, 1638 Wolong Road, Nanyang, Henan, 473061, People's Republic of China. tcd530@126.com.; Postdoctoral Innovation Practice Base, She Dian Lao Jiu Co. Ltd., 2 Liquor Avenue, Nanyang, Henan, 473300, People's Republic of China. tcd530@126.com.; College of Food Science and Technology, Henan Agricultural University, 63 Agricultural Road, Zhengzhou, Henan, 450002, People's Republic of China. tcd530@126.com.
Jazyk: angličtina
Zdroj: Biotechnology for biofuels and bioproducts [Biotechnol Biofuels Bioprod] 2024 Mar 18; Vol. 17 (1), pp. 44. Date of Electronic Publication: 2024 Mar 18.
DOI: 10.1186/s13068-024-02487-4
Abstrakt: 2,5-Dimethylpyrazine (2,5-DMP) is important pharmaceutical raw material and food flavoring agent. Recently, engineering microbes to produce 2,5-DMP has become an attractive alternative to chemical synthesis approach. In this study, metabolic engineering strategies were used to optimize the modified Escherichia coli BL21 (DE3) strain for efficient synthesis of 2,5-DMP using L-threonine dehydrogenase (EcTDH) from Escherichia coli BL21, NADH oxidase (EhNOX) from Enterococcus hirae, aminoacetone oxidase (ScAAO) from Streptococcus cristatus and L-threonine transporter protein (EcSstT) from Escherichia coli BL21, respectively. We further optimized the reaction conditions for synthesizing 2,5-DMP. In optimized conditions, the modified strain can convert L-threonine to obtain 2,5-DMP with a yield of 2897.30 mg/L. Therefore, the strategies used in this study contribute to the development of high-level cell factories for 2,5-DMP.
(© 2024. The Author(s).)
Databáze: MEDLINE
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