A multifunctional fluorescent probe for sequential detection of hydrogen sulfide and pH in foodstuffs, living cells and mice.

Autor: Huang ZS; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, China., Zhang W; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, China., Liang M; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, China., Wang S; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, China., Zhang Z; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, China., Jiang Y; The Affiliated Xiangshan Hospital of Wenzhou Medical University, Xiangshan First People's Hospital Medical and Health Group, Xiangshan, 315700, China., Ye X; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, China. Electronic address: yxx@wmu.edu.cn., Xie L; The Affiliated Xiangshan Hospital of Wenzhou Medical University, Xiangshan First People's Hospital Medical and Health Group, Xiangshan, 315700, China. Electronic address: xfxlt@126.com., Quan YY; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, China. Electronic address: yunyunquan@163.com.
Jazyk: angličtina
Zdroj: Analytica chimica acta [Anal Chim Acta] 2024 Apr 22; Vol. 1299, pp. 342434. Date of Electronic Publication: 2024 Feb 28.
DOI: 10.1016/j.aca.2024.342434
Abstrakt: Background: Cancer as a leading cause of premature death worldwide has become a major threat to human health due to the high incidence and mortality. Monitoring tumor markers are reliable and significantly important for early detection of cancers. In complex biological systems, it is of great urgency but still remains challenging to conceive a fluorescent probe with multiple tumor markers detection property. Hydrogen sulfide (H 2 S) and pH are two target biomarkers for diagnosis of early cancer. The preparation of a novel probe with H 2 S and pH dual detection functions is highly anticipated.
Results: Herein, a novel sequential detection probe HTPQ-HS for H 2 S and pH has been developed. In this system, HPQ (2-(2 -hydroxyphenyl)-4(3H)-quinazolinone) structure combined with triphenylamine is applied as the fluorophore, and 2, 4-dinitrophenylsulfonyl group is used as the recognition group. In the presence of H 2 S, HTPQ-HS is transformed into product HTPQ-OH which shows fluorescence enhancement (29-fold) at 525 nm in less than 4 min and further displays repeatable acid-base responsive ability. HTPQ-HS is able to sequentially response to H 2 S and pH in living cells and does not react directly with pH. Owing to the low cytotoxicity, HTPQ-HS is able to detect exogenous and endogenous H 2 S in colon cancer cells and mice, monitor H 2 S in inflammation model and in foodstuffs. As the environment changes from acidic to alkaline, the fluorescence intensity ratio (I 470 /I 530 ) of product HTPQ-OH changes remarkably, illustrating the ratiometric fluorescent responsiveness to pH.
Significance and Novelty: A multifunctional fluorescent probe HTPQ-HS for sequential detection of H 2 S and pH is synthesized. Probe HTPQ-OH realizes the monitoring of dynamic changes in intracellular pH and displays prospective application in security printing. We expect that our work could offer an important guidance on the development of multifunctional fluorescent probes for visualizing H 2 S and pH in biology and environment.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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