Fabrication and application of salicin-polycaprolactone 3D-printed scaffold in the healing of femur bone defects.

Autor:	Jalali H; Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran., Salemian M; Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran., Nabiuni M; Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran., Kouchesfehani HM; Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran., Bardei LK; Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran., Gregory C; Department of Medical Physiology, Institute for Regenerative Medicine, School of Medicine, Texas A&M University, Texas, United States of America.
Jazyk:	angličtina
Zdroj:	Biomedical materials (Bristol, England) [Biomed Mater] 2024 Mar 28; Vol. 19 (3). Date of Electronic Publication: 2024 Mar 28.
DOI:	10.1088/1748-605X/ad3536
Abstrakt:	Polycaprolactone (PCL) is a suitable material for bone repair due to good biocompatibility and mechanical properties. However, low bioactivity and hydrophobicity pose major challenges for its biomedical applications. To overcome these limitations, PCL-based scaffolds loaded with bioactive agents have been developed. Salicin (Sal) is an anti-inflammatory and analgesic herbal glycoside with osteogenic potential. In the present study, we aimed to produce a Sal-laden PCL (PCL-Sal) scaffold for bone healing applications. Three-dimensional scaffolds were produced and their biocompatibility, and physical-chemical characteristics were determined. The osteogenic potential of the PCL (PCL) and PCL-Sal scaffolds was evaluated using bone marrow mesenchymal stem cells (BMSCs). Scaffolds were implanted into a 5 mm bone defect created in the femur of adult rats, and the new bone fraction was determined using micro-computed tomography scanning at one-month follow-up. PCL-Sal scaffold had a structure, porosity, and fiber diameter suitable for bone construction. It also possessed a higher rate of hydrophilicity and bioactivity compared to the PCL, providing a suitable surface for the proliferation and bone differentiation of BMSCs. Furthermore, PCL-Sal scaffolds showed a higher capacity to scavenge free radicals compared to PCL. The improved bone healing potential of the PCL-Sal scaffold was also confirmed according to in vivo implantation results. Our findings revealed that the Sal-laden implant could be considered for bone repair due to desirable characteristics of Sal such as hydrophilicity, surface modification for cell attachment, and antioxidant properties. (© 2024 IOP Publishing Ltd.)
Databáze:	MEDLINE
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