MbEOMT1 regulates methyleugenol biosynthesis in Melaleuca bracteata F. Muell.

Autor:	Yang C; Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Horticulture, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou 350002, China.; Institute of Natural Products of Horticultural Plants, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou 350002, China., Lin Y; College of Life Sciences, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou 350002, China., Xiang X; Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Horticulture, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou 350002, China.; Institute of Natural Products of Horticultural Plants, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou 350002, China., Shao D; Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Horticulture, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou 350002, China.; Institute of Natural Products of Horticultural Plants, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou 350002, China., Qiu Z; Agricultural Science and Technology Research Center of Chaozhou in Guangdong Province, Qiandong Town, Raoping County, Chaozhou 315600, China., Li Y; Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Horticulture, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou 350002, China.; Institute of Natural Products of Horticultural Plants, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou 350002, China., Wu S; Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Horticulture, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou 350002, China.; Institute of Natural Products of Horticultural Plants, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou 350002, China.
Jazyk:	angličtina
Zdroj:	Tree physiology [Tree Physiol] 2024 Apr 03; Vol. 44 (4).
DOI:	10.1093/treephys/tpae034
Abstrakt:	Methyleugenol, a bioactive compound in the phenylpropene family, undergoes its final and crucial biosynthetic transformation when eugenol O-methyltransferase (EOMT) converts eugenol into methyleugenol. While Melaleuca bracteata F. Muell essential oil is particularly rich in methyleugenol, it contains only trace amounts of its precursor, eugenol. This suggests that the EOMT enzyme in M. bracteata is highly efficient, although it has not yet been characterized. In this study, we isolated and identified an EOMT gene from M. bracteata, termed MbEOMT1, which is primarily expressed in the flowers and leaves and is inducible by methyl jasmonate (MeJA). Subcellular localization of MbEOMT1 in the cytoplasm was detected. Through transient overexpression experiments, we found that MbEOMT1 significantly elevates the concentration of methyleugenol in M. bracteata leaves. Conversely, silencing of MbEOMT1 via virus-induced gene silencing led to a marked reduction in methyleugenol levels. Our in vitro enzymatic assays further confirmed that MbEOMT1 specifically catalyzes the methylation of eugenol. Collectively, these findings establish that the MbEOMT1 gene is critical for methyleugenol biosynthesis in M. bracteata. This study enriches the understanding of phenylpropene biosynthesis and suggests that MbEOMT1 could serve as a valuable catalyst for generating bioactive compounds in the future. (© The Author(s) 2024. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permission@oup.com.)
Databáze:	MEDLINE
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