Determination of carbamazepine in fingerprints: a feasibility study to evaluate adherence in epilepsy patients.

Autor: Müller IR; Analytical Toxicology Laboratory, Universidade Feevale, Rua Rubem Berta, 200, Novo Hamburgo, RS, CEP, 93525-080, Brazil.; Graduate Program on Toxicology and Analytical Toxicology, Universidade Feevale, Rua Rubem Berta, 200, Novo Hamburgo, RS, CEP, 93525-080, Brazil., Grandi VT; College of Medicine, Universidade de Passo Fundo, Rua Teixeira Soares, 817, Passo Fundo, RS, CEP, 99010-080, Brazil., Santos Paulino SG; College of Medicine, Universidade de Passo Fundo, Rua Teixeira Soares, 817, Passo Fundo, RS, CEP, 99010-080, Brazil., Villa B; College of Pharmacy, Universidade de Passo Fundo, Rodovia BR 285, Km 293, Passo Fundo, RS, CEP, 99052-900, Brazil., Zembruski BM; College of Pharmacy, Universidade de Passo Fundo, Rodovia BR 285, Km 293, Passo Fundo, RS, CEP, 99052-900, Brazil., Forcelini CM; Hospital São Vicente de Paulo, Rua Uruguai, 2050, Passo Fundo-RS, CEP, 99010-111, Brazil., Hahn SR; College of Pharmacy, Universidade de Passo Fundo, Rodovia BR 285, Km 293, Passo Fundo, RS, CEP, 99052-900, Brazil., Antunes MV; Analytical Toxicology Laboratory, Universidade Feevale, Rua Rubem Berta, 200, Novo Hamburgo, RS, CEP, 93525-080, Brazil.; Graduate Program on Toxicology and Analytical Toxicology, Universidade Feevale, Rua Rubem Berta, 200, Novo Hamburgo, RS, CEP, 93525-080, Brazil., Linden R; Analytical Toxicology Laboratory, Universidade Feevale, Rua Rubem Berta, 200, Novo Hamburgo, RS, CEP, 93525-080, Brazil.; Graduate Program on Toxicology and Analytical Toxicology, Universidade Feevale, Rua Rubem Berta, 200, Novo Hamburgo, RS, CEP, 93525-080, Brazil.
Jazyk: angličtina
Zdroj: Bioanalysis [Bioanalysis] 2024; Vol. 16 (9), pp. 415-427. Date of Electronic Publication: 2024 Mar 18.
DOI: 10.4155/bio-2023-0265
Abstrakt: Background: Fingerprint drug concentrations can be used as a noninvasive and convenient alternative to evaluate adherence to pharmacotherapy. Methods: Fingerprints were applied over glass slides, extracted and analyzed by ultra-high performance LC-MS/MS. The assay and drug adherence questionnaires were applied to 30 epilepsy patients. Results: The assay had linearity in the range 0.05-10 ng fingerprint -1 , with precision of 2.16-7.9% and accuracy of 95.0-102.8%. Carbamazepine (CBZ) levels in fingerprints were stable at 45°C for 15 days. Concentrations in patient samples were 0.06-9.28 ng fingerprint -1 . A significant difference (p = 0.003) was found between CBZ concentrations in fingerprints between patient groups divided as low and medium/high adherence. Conclusion: This method can potentially be applied to the identification of epilepsy patients with low adherence to CBZ pharmacotherapy.
Databáze: MEDLINE
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