Quantitative pharmacokinetic and biodistribution studies for fluorescent imaging agents.
| Autor: | Feng Y; Geisel School of Medicine, Dartmouth College, 1 Rope Ferry Road, Hanover, NH 03755, USA., Pannem S; Thayer School of Engineering, Dartmouth College, 15 Thayer Drive, Hanover, NH 03755, USA., Hodge S; Thayer School of Engineering, Dartmouth College, 15 Thayer Drive, Hanover, NH 03755, USA., Rounds C; Department of Biomedical Engineering, Illinois Institute of Technology, 10 West 35 Street, Chicago, IL 60616, USA., Tichauer KM; Department of Biomedical Engineering, Illinois Institute of Technology, 10 West 35 Street, Chicago, IL 60616, USA., Paulsen KD; Thayer School of Engineering, Dartmouth College, 15 Thayer Drive, Hanover, NH 03755, USA., Samkoe KS; Geisel School of Medicine, Dartmouth College, 1 Rope Ferry Road, Hanover, NH 03755, USA.; Thayer School of Engineering, Dartmouth College, 15 Thayer Drive, Hanover, NH 03755, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Biomedical optics express [Biomed Opt Express] 2024 Feb 26; Vol. 15 (3), pp. 1861-1877. Date of Electronic Publication: 2024 Feb 26 (Print Publication: 2024). |
| DOI: | 10.1364/BOE.504878 |
| Abstrakt: | Pharmacokinetics and biodistribution studies are essential for characterizing fluorescent agents in vivo . However, few simple methods based on fluorescence imaging are available that account for tissue optical properties and sample volume differences. We describe a method for simultaneously quantifying mean fluorescence intensity of whole blood and homogenized tissues in glass capillary tubes for two fluorescent agents, ABY-029 and IRDye 680LT, using wide-field imaging and tissue-specific calibration curves. All calibration curves demonstrated a high degree of linearity with mean R 2 = 0.99 ± 0.01 and RMSE = 0.12 ± 0.04. However, differences between linear regressions indicate that tissue-specific calibration curves are required for accurate concentration recovery. The lower limit of quantification (LLOQ) for all samples tested was determined to be < 0.3 nM for ABY-029 and < 0.4 nM for IRDye 680LT. Competing Interests: ABY-029 was produced under a National Cancer Institute funded academic-industrial partnership between Dartmouth College, Affibody Medical AB, and LI-COR Biosciences, Inc. (© 2024 Optica Publishing Group.) |
| Databáze: | MEDLINE |
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