Presence of active AKT in the nucleus upon adipocyte differentiation of 3T3-L1 cells.
| Autor: | Goris M; Department of Biological Sciences, University of Bergen, Bergen, Vestland, Norway., Jacobsen RG; Department of Biological Sciences, University of Bergen, Bergen, Vestland, Norway., Lewis AE; Department of Biological Sciences, University of Bergen, Bergen, Vestland, Norway. |
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| Jazyk: | angličtina |
| Zdroj: | MicroPublication biology [MicroPubl Biol] 2024 Feb 29; Vol. 2024. Date of Electronic Publication: 2024 Feb 29 (Print Publication: 2024). |
| DOI: | 10.17912/micropub.biology.001140 |
| Abstrakt: | AKT is an essential player in the phosphoinositide 3-kinase (PI3K) signalling pathway. Although the mechanisms of its action are well understood at the plasma membrane, AKT can also be found in the nucleus. In adipocytes, this pathway is activated during the process of adipogenesis and solicits both plasma membrane and nuclear AKT activity. However, the endogenous presence of active AKT in the nucleus during adipogenesis has not been shown. Here, we show that the levels of active AKT phosphorylated at Ser-473 increase rapidly after the induction of differentiation in 3T3-L1 cells, both in the cytoplasm and in the nucleus, and tend to remain elevated over the course of differentiation. In conclusion, these results support the notion that nuclear AKT plays an important role in this process. Competing Interests: The authors declare that there are no conflicts of interest present. (Copyright: © 2024 by the authors.) |
| Databáze: | MEDLINE |
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