Modulatory effects of Moringa oleifera leaf extract on sodium nitrate-induced experimental colitis via regulation of P53, Ki-67 and PCNA biomarkers.

Autor: Hassan HM; Human Anatomy and Embryology Department, Faculty of Medicine, Mansoura University, Egypt; Human Anatomy and Embryology Department, Faculty of Medicine, New Mansoura University, Egypt. Electronic address: Hendmohammed@mans.edu.eg., Elsaed WM; Human Anatomy and Embryology Department, Faculty of Medicine, Mansoura University, Egypt; Human Anatomy and Embryology Department, National Mansoura University, Gamasa, Egypt. Electronic address: wzaarina@mans.edu.eg., Elzeiny D; Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Mansoura University, Egypt; Department of Medical Biochemistry and Molecular Biology, New Mansoura University, Egypt. Electronic address: dr-dina-ashraf@hotmail.com., Habotta OA; Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Mansoura University, Egypt. Electronic address: ola_ali@mans.edu.eg., Eleraky ES; Department of Internal Medicine, Faculty of Medicine, Horus University, Damietta, Egypt. Electronic address: sheleraky@horus.edu.eg., Nashar EME; Department of Anatomy, College of Medicine, King Khalid University, Abha 62529, Saudi Arabia. Electronic address: enshar@kku.edu.sa., Alghamdi MA; Department of Anatomy, College of Medicine, King Khalid University, Abha 62529, Saudi Arabia; Genomics and Personalized Medicine Unit, The Center for Medical and Health Research, King Khalid University, Abha 62529, Saudi Arabia. Electronic address: m.alghamdi@kku.edu.sa., Aldahhan RA; Department of Anatomy, College of Medicine, Imam Abdulrahman Bin Faisal University, P.O. Box 2114, Dammam 31451, Saudi Arabia. Electronic address: raaldahhan@iau.edu.sa., Alzahrani MA; Internal Medicine Department, College of Medicine, King Khalid University, Abha 62529, Saudi Arabia. Electronic address: malzahrani@kku.edu.sa., Saleh Alamari AM; Gastroenterology Department, Aseer Central Hospital, Abha 62523, Saudi Arabia. Electronic address: Amari0107@hotmail.com., Hamza E; Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Mansoura University, Egypt; Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Horus University, New Damietta, Egypt. Electronic address: ehamza@horus.edu.eg.
Jazyk: angličtina
Zdroj: Tissue & cell [Tissue Cell] 2024 Jun; Vol. 88, pp. 102327. Date of Electronic Publication: 2024 Feb 25.
DOI: 10.1016/j.tice.2024.102327
Abstrakt: Background: Ulcerative colitis is a risk factor for colorectal carcinoma. Different mechanisms are related to colitis like apoptosis and hyperproliferation. Moringa oleifera leaves extract (MO) provides a promising option to overcome the risk.
Purpose: To examine the colonic changes in a rat model of colitis induced by sodium nitrate (SN) and study the effects of MO.
Study Design: Eight adult male rats were allocated in each of the three group; control (distilled water), SN (100 mg/kg/day, orally via gastric gavage), and SN + MO (100 mg/kg/day, orally via gastric gavage).
Methods: Body weight was measured after the end of the experiment. Colonic homogenates were tested for levels of oxidative stress indicators. Immunohistochemistry for P53, PCNA and Ki-67 was performed. Fresh colon specimens were used for quantitative real-time PCR for assessment of P53, PCNA and Ki-67 gene expression.
Results: SN group revealed a significant decreased weight (p = 0.002). MDA and NO levels were higher with SN administration than with MO co-administration (p= 0.04, 0.01 respectively). GSH level was reduced in SN group (p = 0.02) and significantly increased with MO intake (p = 0.04). SN-induced colonic destructive changes were reversed with MO. P53, PCNA and Ki-67 levels of gene expression were reduced in SN + MO group than SN group (P = 0.007, 0.02, 0.001 respectively).
Conclusion: MO protected the colonic mucosa against SN-induced changes regulating apoptosis, and cell proliferation.
Competing Interests: Declaration of Competing Interest The authors have no conflict of interest.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: