Antiprotozoal potential of Vismia species (Hypericaceae), medicinal plants used to fight cutaneous leishmaniasis.

Autor: Diel KAP; Universidade Federal do Rio Grande do Sul, Programa de Pós-Graduação em Ciências Farmacêuticas, Avenida Ipiranga 2752, Santana, 90610-000, Porto Alegre, Rio Grande do Sul, Brazil., Santana Filho PC; Universidade Federal de Ciências da Saúde de Porto Alegre, Departamento de Ciências Básicas da Saúde, Rua Sarmento Leite 245, Centro Histórico, 90050-170, Porto Alegre, Rio Grande do Sul, Brazil., Pitol Silveira P; Universidade Federal do Rio Grande do Sul, Programa de Pós-Graduação em Ciências Farmacêuticas, Avenida Ipiranga 2752, Santana, 90610-000, Porto Alegre, Rio Grande do Sul, Brazil., Ribeiro RL; Universidade Federal de Ciências da Saúde de Porto Alegre, Departamento de Ciências Básicas da Saúde, Rua Sarmento Leite 245, Centro Histórico, 90050-170, Porto Alegre, Rio Grande do Sul, Brazil., Teixeira PC; Universidade Federal de Ciências da Saúde de Porto Alegre, Departamento de Ciências Básicas da Saúde, Rua Sarmento Leite 245, Centro Histórico, 90050-170, Porto Alegre, Rio Grande do Sul, Brazil., Rodrigues Júnior LC; Universidade Federal de Ciências da Saúde de Porto Alegre, Departamento de Ciências Básicas da Saúde, Rua Sarmento Leite 245, Centro Histórico, 90050-170, Porto Alegre, Rio Grande do Sul, Brazil., Marinho LC; Universidade Federal do Maranhão, Departamento de Biologia, Avenida dos Portugueses 1966, Bacanga, 65080-805, São Luís, Maranhão, Brazil., Romão PRT; Universidade Federal de Ciências da Saúde de Porto Alegre, Departamento de Ciências Básicas da Saúde, Rua Sarmento Leite 245, Centro Histórico, 90050-170, Porto Alegre, Rio Grande do Sul, Brazil. Electronic address: pedror@ufcspa.edu.br., von Poser GL; Universidade Federal do Rio Grande do Sul, Programa de Pós-Graduação em Ciências Farmacêuticas, Avenida Ipiranga 2752, Santana, 90610-000, Porto Alegre, Rio Grande do Sul, Brazil. Electronic address: gilsane@farmacia.ufrgs.br.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2024 Jun 28; Vol. 328, pp. 118028. Date of Electronic Publication: 2024 Mar 16.
DOI: 10.1016/j.jep.2024.118028
Abstrakt: Ethnopharmacological Relevance: Species of Vismia (Hypericaceae), known in Brazil as "lacre", are commonly used in traditional Amazonian medicine for the treatment of skin lesions, including those caused by Leishmania infection.
Aim of the Study: Hexane extracts from the leaves of Vismia cayennensis, V. gracilis, V. sandwithii and V. guianensis, as well as from the fruits of the latter, in addition to the anthraquinones vismiaquinone, physcion and chrysophanol isolated from these species were explored for their anti-promastigote and anti-amastigote activity on Leishmania amazonensis.
Materials and Methods: Extracts were prepared by static maceration with n-hexane. The compounds, isolated by chromatographic techniques, were identified by spectroscopic methods ( 1 H and 13 C NMR). Promastigotes of L.amazonensis were incubated with hexane extracts (1-50 μg/mL) or anthraquinones (1-50 μM) and the parasite survival analyzed. The action of compounds on reactive oxygen species (ROS) production, mitochondrial membrane potential, and membrane integrity of promastigotes were evaluated by flow cytometer, and the cytotoxicity on mammalian cells using MTT assay. Furthermore, the activity of compounds against amastigotes and nitric oxide production were also investigated.
Results: Vismiaquinone and physcion were obtained from the leaves of V. guianensis. Physcion, as well as chrysophanol, were isolated from V. sandwithii. Vismia cayennensis and V. gracilis also showed vismiaquinone, compound detected in lower quantity in the fruits of V. guianensis. All extracts were active against the parasite, corroborating the popular use. The greatest activity against promastigotes was achieved with V. guianensis extract (IC 50 4.3 μg/mL), precisely the most used Vismia species for treating cutaneous leishmaniasis. Vismiaquinone and physcion exhibited relevant activity with IC 50 12.6 and 2.6 μM, respectively. Moreover, all extracts and anthraquinones tested induced ROS production, mitochondrial dysfunction, membrane disruption and were able to kill intracellular amastigote forms, being worthy of further in vivo studies as potential antileishmanial drugs.
Conclusions: The overall data achieved in the current investigation scientifically validate the traditional use of Vismia species, mainly V. guianensis, as an anti-Leishmania agent. Furthermore, the promising results presented here indicate species of Vismia as potentially useful resources of Brazilian flora for the discovery of therapeutic solutions for neglected diseases.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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