Protocol for xenografting of BxPC-3 pancreatic tumors using a chorioallantoic membrane model.

Autor:	Colenbier R; Laboratory of Cell Biology and Histology, Faculty of Medicine and Health Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, 2610 Antwerp, Belgium. Electronic address: robin.colenbier@uantwerpen.be., Coppens L; Laboratory of Cell Biology and Histology, Faculty of Medicine and Health Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, 2610 Antwerp, Belgium., Logghe T; ElmediX NV, 2800 Mechelen, Belgium., van Zwol E; ElmediX NV, 2800 Mechelen, Belgium., Timmermans JP; Laboratory of Cell Biology and Histology, Faculty of Medicine and Health Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, 2610 Antwerp, Belgium., Bogers J; Laboratory of Cell Biology and Histology, Faculty of Medicine and Health Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, 2610 Antwerp, Belgium; ElmediX NV, 2800 Mechelen, Belgium. Electronic address: john-paul.bogers@uantwerpen.be.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2024 Jun 21; Vol. 5 (2), pp. 102968. Date of Electronic Publication: 2024 Mar 15.
DOI:	10.1016/j.xpro.2024.102968
Abstrakt:	The chorioallantoic membrane (CAM) model is an increasingly attractive model for the study of human tumors. However, concise techniques for the use of pancreatic ductal adenocarcinoma BxPC-3 xenografts in CAM assays are not yet available. Here, we present a protocol for the induction of BxPC-3 xenograft tumors with high grafting efficiency. We describe steps for embryo incubation, egg handling, and grafting, each of which has been optimized to prevent fungal contamination and minimize mortality. Competing Interests: Declaration of interests This research is partly funded by a grant from ElmediX NV. T.L. and E.v.Z. are employees of ElmediX. J.B. is the CEO and a shareholder of ElmediX NV. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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