Structural Changes in Rat Hepatocyte Nucleolus under Nucleolar Stress Caused by Hypothermia.

Autor: Bobrov IP; Altai State Medical University, Ministry of Health of the Russian Federation, Barnaul, Russia., Dolgatov AY; Altai State Medical University, Ministry of Health of the Russian Federation, Barnaul, Russia., Lepilov AV; Altai State Medical University, Ministry of Health of the Russian Federation, Barnaul, Russia., Korsikov NA; Altai State Medical University, Ministry of Health of the Russian Federation, Barnaul, Russia., Dolgatova ES; Altai State Medical University, Ministry of Health of the Russian Federation, Barnaul, Russia., Klinnikova MG; Institute of Molecular Pathology and Pathomorphology, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia., Lushnikova EL; Institute of Molecular Pathology and Pathomorphology, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia. pathol@inbox.ru.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2024 Feb; Vol. 176 (4), pp. 519-522. Date of Electronic Publication: 2024 Mar 16.
DOI: 10.1007/s10517-024-06059-2
Abstrakt: Structural changes in rat hepatocyte nucleoli were studied during deep hypothermia simulated by immersion in water at 5°C for 40 min (ambient air temperature 7°C). In comparison with the control, phenomena of nucleolar stress occurred in rats during hypothermia: the number of fibrillar centers (FC) per nucleus (by 1.7 times) and per nucleolus (by 1.6 times), nucleolonemal nucleoli per nucleus (by 2.8 times), and the relative content of nucleolonemal nucleoli per nucleus (by 2.6 times) significantly decreased (p=0.0000001); the number of FC per nucleolonemal nucleolus also decreased by 1.4 times (p=0.01). In the hepatocyte nuclei, we observed an increase in the relative content of transitional type nucleoli per nucleus (by 1.3 times; p=0.01), the number of FC per transitional type nucleolus (by 1.4 times; p=0.003), the content of free FC per nucleus (by 3 times; p=0.00004), and the percentage of free FC per nucleus (by 3.5 times; p=0.00004). These changes can be considered as compensatory and adaptive reactions, and transitional type nucleoli can be attributed to the "reserve" nucleolar pool.
(© 2024. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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