Proliferative Effects of Mesenchymal Stromal Cells on Neuroblastoma Cell Lines: Are They Tumor Promoting or Tumor Inhibiting?

Autor: Doyle K; Department of Surgery, University of California-Davis, Sacramento, CA, USA. Electronic address: kedoyle@ucdavis.edu., Sutter M; Center for Surgical Bioengineering, Department of Surgery, University of California-Davis, Sacramento, CA, USA., Rodriguez M; Center for Surgical Bioengineering, Department of Surgery, University of California-Davis, Sacramento, CA, USA., Hassan AE; Department of Surgery, University of California-Davis, Sacramento, CA, USA., Kumar P; Center for Surgical Bioengineering, Department of Surgery, University of California-Davis, Sacramento, CA, USA., Brown E; Department of Surgery, Division of Pediatric Surgery, University of California-Davis, Sacramento, CA, USA.
Jazyk: angličtina
Zdroj: Journal of pediatric surgery [J Pediatr Surg] 2024 Aug; Vol. 59 (8), pp. 1582-1590. Date of Electronic Publication: 2024 Feb 26.
DOI: 10.1016/j.jpedsurg.2024.02.014
Abstrakt: Background: Neuroblastoma is a common pediatric malignancy with poor survival for high-risk disease. Mesenchymal stromal cells (MSCs) have innate tumor-homing properties, enabling them to serve as a cellular delivery vehicle, but MSCs have demonstrated variable effects on tumor growth. We compared how placental MSCs (PMSCs) and bone marrow-derived MSCs (BM-MSCs) affect proliferation of neuroblastoma (NB) cells in vitro.
Methods: Indirect co-culture assessed proliferative effects of 18 MSCs (early-gestation PMSCs (n = 9), term PMSCs (n = 5), BM-MSCs (n = 4) on three high-risk NB cell lines (NB1643, SH-SY5Y, and CHLA90). Controls were NB cells cultured in media alone. Proliferation was assessed using MTS assay and measured by fold change (fc) over controls. PMSCs were sub-grouped by neuroprotective effect: strong (n = 7), intermediate (n = 3), and weak (n = 4). The relationship between MSC type, PMSC neuroprotection, and PMSC gestational age on NB cell proliferation was assessed.
Results: NB cell proliferation varied between MSC groups. BM-MSCs demonstrated lower proliferative effects than PMSCs (fc 1.18 vs 1.44, p < 0.001). Neither gestational age nor neuroprotection significantly predicted degree of proliferation. Proliferative effects of MSCs varied among NB cell lines. BM-MSCs had less effect on CHLA90 (fc 1.01) compared to NB1643 (fc 1.33) and SH-SY5Y (fc 1.20). Only NB1643 showed a difference between early and term PMSCs (p = 0.04).
Conclusion: Effects of MSCs on NB cell proliferation vary by MSC source and NB cell line. BM-MSCs demonstrated lower proliferative effects than most PMSCs. MSC neuroprotection was not correlated with proliferation. Improved understanding of MSC proliferation-promoting mechanisms may provide valuable insight into selection of cells best suited as drug delivery vehicles.
Level of Evidence: N/A.
Type of Study: Original Research.
(Published by Elsevier Inc.)
Databáze: MEDLINE
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